Analyte detection using an active assay
- Manassas, VA
- Cross Junction, VA
- Potomac Falls, VA
Analytes using an active assay may be detected by introducing an analyte solution containing a plurality of analytes to a lacquered membrane. The lacquered membrane may be a membrane having at least one surface treated with a layer of polymers. The lacquered membrane may be semi-permeable to nonanalytes. The layer of polymers may include cross-linked polymers. A plurality of probe molecules may be arrayed and immobilized on the lacquered membrane. An external force may be applied to the analyte solution to move the analytes towards the lacquered membrane. Movement may cause some or all of the analytes to bind to the lacquered membrane. In cases where probe molecules are presented, some or all of the analytes may bind to probe molecules. The direction of the external force may be reversed to remove unbound or weakly bound analytes. Bound analytes may be detected using known detection types.
- Research Organization:
- George Mason Intellectual Properties, Inc. (Fairfax, VA)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- FC52-04NA25455
- Assignee:
- George Mason Intellectual Properties, Inc. (Fairfax, VA)
- Patent Number(s):
- 7,824,927
- Application Number:
- US Patent Application 11/397,905
- OSTI ID:
- 1014958
- Country of Publication:
- United States
- Language:
- English
Similar Records
Solution phase nucleic acid sandwich assay
Quenching methods for background reduction in luminescence-based probe-target binding assays