Modulation of expression of genes encoding nuclear proteins following exposure to JANUS neutrons or {gamma}-rays
- Loyola Univ. Medical Center, Maywood, IL (United States)
- Argonne National Lab., IL (United States)
Previous work has shown that exposure of cells to ionizing radiations causes modulation of a variety of genes, including those encoding c-fos, interleukin-1, tumor necrosis factor, and cytoskeletal elements. The experiments reported herein were designed to examine the effects of either JANUS neutron or {gamma}-ray exposure on expression of genes encoding nucleus-associated proteins (H4-histone, c-jun, c-myc, Rb, and p53). Cycling Syrian hamster embryo cells were irradiated with varying doses and dose rates of either JANUS fission-spectrum neutrons or {gamma}-rays; after incubation of the cell cultures for 1 h following radiation exposure, mRNA was harvested and analyzed by Northern blot. Results revealed induction of transcripts for c-jun, H4-histone, and (to a lesser extent) Rb following {gamma}-ray but not following neutron exposure. Expression of p53 and c-myc genes was unaffected by radiation exposure. Radiations at different doses and dose rates were compared for each of the genes studied.
- Research Organization:
- Argonne National Lab. (ANL), Argonne, IL (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- W-31109-ENG-38
- OSTI ID:
- 10148904
- Report Number(s):
- ANL/BIM/PP-78237; ON: DE94011487
- Resource Relation:
- Other Information: PBD: [1994]
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
FISSION NEUTRONS
RADIOBIOLOGY
ONCOGENES
GENE REGULATION
HISTONES
ENZYME INDUCTION
PHOSPHOTRANSFERASES
COBALT 60
CHO CELLS
GAMMA RADIATION
CELL CYCLE
560120
550200
RADIATION EFFECTS ON BIOCHEMICALS, CELLS, AND TISSUE CULTURE
BIOCHEMISTRY