Repair of mismatched basepairs in mammalian DNA. Progress report, March 1, 1990--February 28, 1991
We have concentrated on three specific areas of our research plan. Our greatest emphasis is on the role of single strand nicks in influencing template strand selection in mismatch repair. We have found, that the ability of a nick in one strand to influence which strand is repaired is not a simple function of distance from the mismatched site but rather that an hot spot where a nick is more likely to have an influence can exist. The second line was production of single-genotype heteroduplexes in order to examine independently the repair of T/G and A/C mispairs within the same sequence context as in our mixed mispair preparations. We have shown preparations of supercoiled heteroduplex can be prepared that were exclusively T/G or exclusively A/C at the mispair site. The third effort has been to understand the difference in repair bias of different cell lines or different transfection conditions as it may relate to different repair systems in the cell. We have identified some of the sources of variation, including cell cycle position. We hope to continue this work to more precisely identify the phase of the cell cycle.
- Research Organization:
- Florida State Univ., Tallahassee, FL (United States). Inst. of Molecular Biophysics
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG05-87ER60545
- OSTI ID:
- 10146510
- Report Number(s):
- DOE/ER/60545-4; ON: DE93013262
- Resource Relation:
- Other Information: PBD: Aug 1991
- Country of Publication:
- United States
- Language:
- English
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
DNA MISMATCH
DNA REPAIR
PROGRESS REPORT
DISTANCE
OLIGONUCLEOTIDES
SIMIAN VIRUS
CELL CYCLE
ANIMAL CELLS
550200
560120
BIOCHEMISTRY
RADIATION EFFECTS ON BIOCHEMICALS, CELLS, AND TISSUE CULTURE