High resolution mass spectrometry method and system for analysis of whole proteins and other large molecules
- Knoxville, TN
- Naperville, IL
A matrix assisted laser desorption/ionization (MALDI) method and related system for analyzing high molecular weight analytes includes the steps of providing at least one matrix-containing particle inside an ion trap, wherein at least one high molecular weight analyte molecule is provided within the matrix-containing particle, and MALDI on the high molecular weight particle while within the ion trap. A laser power used for ionization is sufficient to completely vaporize the particle and form at least one high molecular weight analyte ion, but is low enough to avoid fragmenting the high molecular weight analyte ion. The high molecular weight analyte ion is extracted out from the ion trap, and is then analyzed using a detector. The detector is preferably a pyrolyzing and ionizing detector.
- Research Organization:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-00OR22725
- Assignee:
- UT-Battelle, LLC (Oak Ridge, TN)
- Patent Number(s):
- 7,671,330
- Application Number:
- US Patent Application 11/688,996
- OSTI ID:
- 1014525
- Country of Publication:
- United States
- Language:
- English
Aerosol MALDI of peptides and proteins in an ion trap mass spectrometer: Trapping, resolution and signal-to-noise
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journal | December 2006 |
MALDI of Individual Biomolecule-Containing Airborne Particles in an Ion Trap Mass Spectrometer
|
journal | July 2005 |
A digital ion trap mass spectrometer coupled with atmospheric pressure ion sources
|
journal | May 2004 |
Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons
|
journal | October 1988 |
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