3-dimensional imaging at nanometer resolutions

Patent · Tue Mar 09 00:00:00 EST 2010

OSTI ID:1014498

Werner, James H ^[1]; Goodwin, Peter M ^[1]; Shreve, Andrew P ^[2]

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1. Los Alamos, NM
2. Santa Fe, NM

An apparatus and method for enabling precise, 3-dimensional, photoactivation localization microscopy (PALM) using selective, two-photon activation of fluorophores in a single z-slice of a sample in cooperation with time-gated imaging for reducing the background radiation from other image planes to levels suitable for single-molecule detection and spatial location, are described.

• Research Organization: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC52-06NA25396
• Assignee: Los Alamos National Security, LLC (Los Alamos, NM)
• Patent Number(s): 7,675,045
• Application Number: U S Patent Application 12/248,834
• OSTI ID: 1014498
• Country of Publication: United States
• Language: English