Biosurfactants and increased bioavailability of sorbed organic contaminants: Measurements using a biosensor

Conference · Fri Dec 31 00:00:00 EST 1993

OSTI ID:10137531

Strong-Gunderson, J M; Palumbo, A V ^[1]; Applegate, B; Saylor, G S ^[2]

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1. Oak Ridge National Lab., TN (United States)
2. Tennessee Univ., Knoxville, TN (United States)

Bioremediation of sites contaminated with hydrophobic materials that sorb onto the soil matrix is very difficult due to reduced microbial (bio)availability. Following biosurfactant addition, we have measured an increase in contaminant bioavailability by using a lux biosensor. Direct microbial bioavailability was determined by using a genetically engineered microbial bioreporter strain of Pseudomonas putida. This strain was engineered so the lux genes, which code for light production, are transcriptionally fused with genes that code for contaminant degradation and are thus induced in the presence of specific compounds. By using a bioreporter we can quantify the actual microbial bioavailability of the contaminants and compare it to concentrations measured by other analytical methods (e.g. gas chromatograph). It is possible that these values are not equal to each other. Thus, bioremediation rates may not be accurately predicted if bioavailability is not considered.

• Research Organization: Martin Marietta Energy Systems, Inc., Oak Ridge, TN (United States); Tennessee Univ., Knoxville, TN (United States)
• Sponsoring Organization: USDOE, Washington, DC (United States); Department of Defense, Washington, DC (United States)
• DOE Contract Number: AC05-84OR21400; FG05-91ER61193
• OSTI ID: 10137531
• Report Number(s): CONF-931136-3; ON: DE94009161; BR: KP0203000; CNN: F4962092-J-0147; TRN: 94:011948
• Resource Relation: Conference: 6. conference on gas, oil and environmental biotechnology,Colorado Springs, CO (United States),29 Nov - 1 Dec 1993; Other Information: PBD: [1993]
• Country of Publication: United States
• Language: English

Related Subjects

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE WASTES FROM NUCLEAR FACILITIES
11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS
54 ENVIRONMENTAL SCIENCES
SOILS
WASHING
ENVIRONMENT
BIOLOGICAL RECOVERY
BIODEGRADATION
HAZARDOUS MATERIALS
REMEDIAL ACTION
MICROORGANISMS
PSEUDOMONAS
GROUND WATER
WASTE MANAGEMENT
SOLUBILITY
BENCH-SCALE EXPERIMENTS
BIOLUMINESCENCE
052000
054000
540220
HEALTH AND SAFETY
CHEMICALS MONITORING AND TRANSPORT