Regulation of the synthesis and assembly of the plant vacuolar H{sup +}-ATPase. Progress report, [April 1, 1991--March 31, 1992]

Technical Report · Wed Apr 01 00:00:00 EST 1992

DOI:https://doi.org/10.2172/10135025· OSTI ID:10135025

Taiz, L

During the past three years we have focused on four main areas: the characterization of the 5{prime}-upstream sequence of the gene for the V-ATPase 70 kDa (A) subunit gene, the generation of V-ATPase-deficient mutants using antisense constructs of the A subunit cDNA, analysis of V-ATPase ultrastructure by negative staining and the characterization of organelle-specific isoforms of the A subunit of carrot. In addition we have extended our studies on the cellular distribution of the V-ATPase and we have continued our investigation of the evolution of the V-ATPases by characterizing the A and B subunits of two species of the archaebacterium, Methanococcus.

• Research Organization: California Univ., Santa Cruz, CA (United States). Dept. of Biology
• Sponsoring Organization: USDOE, Washington, DC (United States)
• DOE Contract Number: FG03-84ER13245
• OSTI ID: 10135025
• Report Number(s): DOE/ER/13245-T1; ON: DE92010627
• Resource Relation: Other Information: PBD: [1992]
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CARROTS
ATP-ASE
BACTERIA
RESEARCH PROGRAMS
PROGRESS REPORT
MUTATIONS
DNA SEQUENCING
CLONING
TRANSCRIPTION
DISTRIBUTION
MITOCHONDRIA
550200
550400
BIOCHEMISTRY
GENETICS