Computer assisted multiplex sequencing. Performance report, August 1, 1992--July 15, 1993
The objectives of this project are automation for optimization of multiplex sequencing. We have integrated direct transfer electrophoresis, automated multiplex hybridizations and automated film reading and applied this toward sequencing of E. coli and human DNA. Primers for the directed dideoxy sequence walking and sequence confirmation steps are synthesized to include DNA tags complementary to an alkaline phosphatase conjugate. A higher throughput synthesis device is well along in testing as are new automated hybridization devices. We have developed software for automatically annotating ORFs and databases of precise termini of proteins and RNA.
- Research Organization:
- Harvard Medical School, Boston, MA (United States). Dept. of Genetics
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG02-87ER60565
- OSTI ID:
- 10135003
- Report Number(s):
- DOE/ER/60565-7; ON: DE94008549; BR: HA0202020/KP0404000
- Resource Relation:
- Other Information: PBD: Jul 1993
- Country of Publication:
- United States
- Language:
- English
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