Biologically important radiation damage in DNA. Annual progress report, May 1, 1993--January 31, 1994

Technical Report · Tue Mar 01 00:00:00 EST 1994

DOI:https://doi.org/10.2172/10134277· OSTI ID:10134277

Ward, J F

Most DNA damage by the hydroxyl radical is confined to the bases, and this base damage represents an important component of locally multiply demanded sites (LMOS). The yields of the major damaged bases have been determined by gas chromatography mass spectrometry. For our propose, it was necessary to convert a known fraction of these damaged bases to strand breaks and then assay these labile sites as the increase in strand break yield over the normally observed level. Three potential agents by which this strategy of conversion of base damage to strand break could be implemented were identified in the original application: 1, Sl nuclease; 2, piperidine; and 3, base damage specific enzymes.

• Research Organization: California Univ., San Diego, La Jolla, CA (United States)
• Sponsoring Organization: USDOE, Washington, DC (United States)
• DOE Contract Number: FG03-93ER61592
• OSTI ID: 10134277
• Report Number(s): DOE/ER/61592-T1; ON: DE94008498; BR: KP0303000; TRN: 94:006506
• Resource Relation: Other Information: PBD: [1994]
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
DNA
RADIOLYSIS
NUCLEASES
PROGRESS REPORT
ANIONS
HYDROXYL RADICALS
DMSO
PIPERIDINES
ENZYME ACTIVITY
560120
RADIATION EFFECTS ON BIOCHEMICALS, CELLS, AND TISSUE CULTURE