High resolution three dimensional microscopy of biological microstructures using zone plate lenses with x-ray laser illumination
One of the goals of biomedical research is the development of imaging techniques capable of producing high resolution ({approximately}300{Angstrom}) three dimensional images of structures within live cells. Recent developments in zone plate lenses at LBL and in x-ray lasers at LLNL indicate that flash three dimensional x-ray microscopy of live biological objects can be achieved in the near term. This concept for a microscope utilizes an x-ray laser to backlit immunogold labeled biological objects. These backlit objects are then imaged with low f-number, high resolution zone plate lenses. Backlighting and imaging along several different directions allows a three dimensional image to be obtained using tomographic techniques.
- Research Organization:
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 10118450
- Report Number(s):
- UCRL-ID-105823; ON: DE94005955
- Resource Relation:
- Other Information: PBD: 12 Dec 1990
- Country of Publication:
- United States
- Language:
- English
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