Microbial strain improvement for organosulfur removal from coal. Final technical report, September 1, 1991--August 31, 1992
- Institute of Gas Technology, Chicago, IL (United States)
IGT has developed a microbial culture of Rhodococcus rhodochrous, designated as IGTS8, that is capable of specifically cleaving carbon-sulfur bonds in a range of organosulfur model compounds and is capable of removing organic sulfur from coal and petroleum without significantly sacrificing the calorific value of the fuel. Although IGTS8 possesses the ability to specifically remove organic sulfur from coal, a major research need is to develop improved strains of microorganisms that possess higher levels of desulfurization activity and therefore will permit more favorable biodesulfurization process conditions: faster rates, more complete removal, and smaller reactor size. Strain improvement is the single most important aspect to the development of a practical coal biodesulfurization process and accordingly is the focus of research in this project. The first steps in obtaining high levels of expression of desulfurization genes are to identify specific DNA fragments that encode desulfurization genes and to identify a DNA fragment that encodes a promoter/regulatory element that is capable of functioning at high levels in Rhodococcus rhodochrous. Both of these goals have been accomplished during the first year of this two-year research program.
- Research Organization:
- Illinois Dept. of Energy and Natural Resources, Springfield, IL (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States); Illinois Dept. of Energy and Natural Resources, Springfield, IL (United States)
- DOE Contract Number:
- FG22-91PC91334
- OSTI ID:
- 10117805
- Report Number(s):
- DOE/PC/91334-T130; ON: DE94005731; BR: AV0100000
- Resource Relation:
- Other Information: PBD: [1992]
- Country of Publication:
- United States
- Language:
- English
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