Unravelling lignin formation and structure. Final report, April 1, 1988--March 31, 1991
- Washington State Univ., Pullman, WA (United States). Inst. of Biological Chemistry
During this study, we established that the Fagaceae exclusively accumulate Z-monolignois/glucosides, and not the E-isomers. Evidence for the presence of a novel E{yields}Z isomerse has been obtained. Our pioneering work in lignin biosynthesis and structure in situ has also progressed smoothly. We established the bonding environments of a woody angiosperm, Leucanea leucocephala, as well as wheat (T. aestivum) and tobacco (N. tabacum). A cell culture system from Pinus taeda was developed which seems ideal for investigating the early stages of lignification. These cultures excrete peroxidase isozymes, considered to be specifically involved in lignin deposition. We also studied the effect of the putative lignin-degrading enzyme, lignin peroxidase, on monolignols and dehydropolymerisates therefrom. In all cases, polymerization was observed, and not degradation; these polymers are identical to that obtained with horseradish peroxidases/H{sub 2}O{sub 2}. It seems inconceivable that these enzymes can be considered as being primarily responsible for lignin biodegradation.
- Research Organization:
- Virginia Polytechnic Inst. and State Univ., Blacksburg, VA (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG05-88ER13883
- OSTI ID:
- 10116727
- Report Number(s):
- DOE/ER/13883-T1; ON: DE92006442
- Resource Relation:
- Other Information: PBD: [1991]
- Country of Publication:
- United States
- Language:
- English
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