U.S. Department of EnergyOffice of Scientific and Technical Information
Genetic variation in resistance to ionizing radiation. Progress report, January--July 1990
Abstract
We proposed an investigation of genetically-determined individual differences in sensitivity to ionizing radiation. The model organism is Drosophila melanogaster. The gene coding for Cu, Zn superoxide dismutase (SOD) is the target locus, but the effects of variation in other components of the genome that modulate SOD levels are also taken into account. SOD scavenges oxygen radicals generated during exposure to ionizing radiation. It has been shown to protect against ionizing radiation damage to DNA, viruses, bacteria, mammalian cells, whole mice, and Drosophila. Two alleles, S and F, are commonly found in natural populations of D. melanogaster; in addition we have isolated from a natural population of ``null`` (CA1) mutant that yields only 3.5% of normal SOD activity. The S, F, and CA1 alleles provide an ideal model system to investigate SOD-dependent radioresistance, because each allele yields different levels of SOD, so that S > F >> CA1. The role of SOD levels in radioresistance are being investigated in a series of experiments that measure the somatic and germ-line effects of increasing doses of ionizing radiation. During the first seven months of funding we have completed a number of experiments and are proceeding with many others. We have made progress alongmore »
- Authors:
- Publication Date:
- Research Org.:
- California Univ., Irvine, CA (United States). Dept. of Ecology and Evolutionary Biology
- Sponsoring Org.:
- USDOE, Washington, DC (United States)
- OSTI Identifier:
- 10116222
- Report Number(s):
- DOE/ER/60713-T2
ON: DE92007059
- DOE Contract Number:
- FG03-88ER60713
- Resource Type:
- Technical Report
- Resource Relation:
- Other Information: PBD: [1990]
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; DROSOPHILA; GENETIC RADIATION EFFECTS; PROGRESS REPORT; GENETIC VARIABILITY; IONIZING RADIATIONS; ENZYMES; RADICALS; BIOCHEMISTRY; OXYGEN; COPPER COMPOUNDS; ZINC COMPOUNDS; GENOTYPE; ENZYME ACTIVITY; MUTANTS; STERILITY; 560152; 550400; ANIMALS; GENETICS
Citation Formats
Ayala, F. J. Genetic variation in resistance to ionizing radiation. Progress report, January--July 1990. United States: N. p., 1990.
Web. doi:10.2172/10116222.
Ayala, F. J. Genetic variation in resistance to ionizing radiation. Progress report, January--July 1990. United States. https://doi.org/10.2172/10116222
Ayala, F. J. 1990.
"Genetic variation in resistance to ionizing radiation. Progress report, January--July 1990". United States. https://doi.org/10.2172/10116222. https://www.osti.gov/servlets/purl/10116222.
@article{osti_10116222,
title = {Genetic variation in resistance to ionizing radiation. Progress report, January--July 1990},
author = {Ayala, F. J.},
abstractNote = {We proposed an investigation of genetically-determined individual differences in sensitivity to ionizing radiation. The model organism is Drosophila melanogaster. The gene coding for Cu, Zn superoxide dismutase (SOD) is the target locus, but the effects of variation in other components of the genome that modulate SOD levels are also taken into account. SOD scavenges oxygen radicals generated during exposure to ionizing radiation. It has been shown to protect against ionizing radiation damage to DNA, viruses, bacteria, mammalian cells, whole mice, and Drosophila. Two alleles, S and F, are commonly found in natural populations of D. melanogaster; in addition we have isolated from a natural population of ``null`` (CA1) mutant that yields only 3.5% of normal SOD activity. The S, F, and CA1 alleles provide an ideal model system to investigate SOD-dependent radioresistance, because each allele yields different levels of SOD, so that S > F >> CA1. The role of SOD levels in radioresistance are being investigated in a series of experiments that measure the somatic and germ-line effects of increasing doses of ionizing radiation. During the first seven months of funding we have completed a number of experiments and are proceeding with many others. We have made progress along all the research lines anticipated for the first year of this grant, as summarized in the following pages.},
doi = {10.2172/10116222},
url = {https://www.osti.gov/biblio/10116222},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Dec 31 00:00:00 EST 1990},
month = {Mon Dec 31 00:00:00 EST 1990}
}