Acute toxicity screening of Hanford Site waste grouts using aquatic invertebrates
Waste grouts prepared by mixing a simulated nonradioactive liquid waste with a dry solids blend consisting of cement, fly ash, and clay were screened for their acute toxicity using aquatic invertebrates (D. magna, D. pulex, and C. dubia) as test organisms and a fluorogenic substrate (4-methylumbelliferyl b-d galactoside) as the toxic stress indicator. After one hour of exposing juvenile daphnids to grout extracts of varying concentrations, followed by a 15-minute reaction with the fluorogenic substrate, the degree of in vivo enzymatic inhibition was measured by the number of resulting fluorescent daphnids. The effective concentration at which 50% of the daphnids were adversely affected (EC50) values calculated by probit analysis were 2,877 mg/L, 2,983 mg/L, and 3,174 mg/L for D. pulex, D. magna, and C. dubia, respectively. The results indicated that the grout extracts studied are nonhazardous and not dangerous to daphnids.
- Research Organization:
- Westinghouse Hanford Co., Richland, WA (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- AC06-87RL10930
- OSTI ID:
- 10112074
- Report Number(s):
- WHC-SA-1927; CONF-931152-1; ON: DE94004823; BR: 35AF11201/35AF11202; TRN: 94:008414
- Resource Relation:
- Conference: 14. annual meeting of the Society of Environmental Toxicology and Chemistry (SETAC),Houston, TX (United States),14-18 Nov 1993; Other Information: PBD: Nov 1993
- Country of Publication:
- United States
- Language:
- English
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