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Title: MCF-10A-NeoST: A New Cell System for Studying Cell-ECM and Cell-Cell Interactions in Breast Cancer

Abstract

There is a continuing need for genetically matched cell systems to model cellular behaviors that are frequently observed in aggressive breast cancers. We report here the isolation and initial characterization of a spontaneously arising variant of MCF-10A cells, NeoST, which provides a new model to study cell adhesion and signal transduction in breast cancer. NeoST cells recapitulate important biological and biochemical features of metastatic breast cancer, including anchorage-independent growth, invasiveness in threedimensional reconstituted membranes, loss of E-cadherin expression, and increased tyrosine kinase activity. A comprehensive analysis of tyrosine kinase expression revealed overexpression or functional activation of the Axl, FAK, and EphA2 tyrosine kinases in transformed MCF-10A cells. MCF-10A and these new derivatives provide a genetically matched model to study defects in cell adhesion and signaling that are relevant to cellular behaviors that often typify aggressive breast cancer cells.

Authors:
; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
Life Sciences Division
OSTI Identifier:
1009837
Report Number(s):
LBNL-4337E
Journal ID: 1078-0432; TRN: US201106%%1027
DOE Contract Number:  
DE-AC02-05CH11231; CA85615, CA57621, CA64786, DE-AC03-76SF00098
Resource Type:
Journal Article
Journal Name:
Clinical Cancer Research
Additional Journal Information:
Journal Volume: 7; Journal Issue: 11; Related Information: Journal Publication Date: 11/01/2001
Country of Publication:
United States
Language:
English
Subject:
59; ADHESION; DEFECTS; FUNCTIONALS; MAMMARY GLANDS; MEMBRANES; NEOPLASMS; PHOSPHOTRANSFERASES; TYROSINE

Citation Formats

Zantek, Nicole Dodge, Walker-Daniels, Jennifer, Stewart, Jane, Hansen, Rhonda K, Robinson, Daniel, Miao, Hui, Wang, Bingcheng, Kung, Hsing-Jien, Bissell, Mina J, and Kinch, Michael S. MCF-10A-NeoST: A New Cell System for Studying Cell-ECM and Cell-Cell Interactions in Breast Cancer. United States: N. p., 2001. Web.
Zantek, Nicole Dodge, Walker-Daniels, Jennifer, Stewart, Jane, Hansen, Rhonda K, Robinson, Daniel, Miao, Hui, Wang, Bingcheng, Kung, Hsing-Jien, Bissell, Mina J, & Kinch, Michael S. MCF-10A-NeoST: A New Cell System for Studying Cell-ECM and Cell-Cell Interactions in Breast Cancer. United States.
Zantek, Nicole Dodge, Walker-Daniels, Jennifer, Stewart, Jane, Hansen, Rhonda K, Robinson, Daniel, Miao, Hui, Wang, Bingcheng, Kung, Hsing-Jien, Bissell, Mina J, and Kinch, Michael S. 2001. "MCF-10A-NeoST: A New Cell System for Studying Cell-ECM and Cell-Cell Interactions in Breast Cancer". United States. https://www.osti.gov/servlets/purl/1009837.
@article{osti_1009837,
title = {MCF-10A-NeoST: A New Cell System for Studying Cell-ECM and Cell-Cell Interactions in Breast Cancer},
author = {Zantek, Nicole Dodge and Walker-Daniels, Jennifer and Stewart, Jane and Hansen, Rhonda K and Robinson, Daniel and Miao, Hui and Wang, Bingcheng and Kung, Hsing-Jien and Bissell, Mina J and Kinch, Michael S},
abstractNote = {There is a continuing need for genetically matched cell systems to model cellular behaviors that are frequently observed in aggressive breast cancers. We report here the isolation and initial characterization of a spontaneously arising variant of MCF-10A cells, NeoST, which provides a new model to study cell adhesion and signal transduction in breast cancer. NeoST cells recapitulate important biological and biochemical features of metastatic breast cancer, including anchorage-independent growth, invasiveness in threedimensional reconstituted membranes, loss of E-cadherin expression, and increased tyrosine kinase activity. A comprehensive analysis of tyrosine kinase expression revealed overexpression or functional activation of the Axl, FAK, and EphA2 tyrosine kinases in transformed MCF-10A cells. MCF-10A and these new derivatives provide a genetically matched model to study defects in cell adhesion and signaling that are relevant to cellular behaviors that often typify aggressive breast cancer cells.},
doi = {},
url = {https://www.osti.gov/biblio/1009837}, journal = {Clinical Cancer Research},
number = 11,
volume = 7,
place = {United States},
year = {Wed Aug 22 00:00:00 EDT 2001},
month = {Wed Aug 22 00:00:00 EDT 2001}
}