MCF-10A-NeoST: A New Cell System for Studying Cell-ECM and Cell-Cell Interactions in Breast Cancer
Abstract
There is a continuing need for genetically matched cell systems to model cellular behaviors that are frequently observed in aggressive breast cancers. We report here the isolation and initial characterization of a spontaneously arising variant of MCF-10A cells, NeoST, which provides a new model to study cell adhesion and signal transduction in breast cancer. NeoST cells recapitulate important biological and biochemical features of metastatic breast cancer, including anchorage-independent growth, invasiveness in threedimensional reconstituted membranes, loss of E-cadherin expression, and increased tyrosine kinase activity. A comprehensive analysis of tyrosine kinase expression revealed overexpression or functional activation of the Axl, FAK, and EphA2 tyrosine kinases in transformed MCF-10A cells. MCF-10A and these new derivatives provide a genetically matched model to study defects in cell adhesion and signaling that are relevant to cellular behaviors that often typify aggressive breast cancer cells.
- Authors:
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- Life Sciences Division
- OSTI Identifier:
- 1009837
- Report Number(s):
- LBNL-4337E
Journal ID: 1078-0432; TRN: US201106%%1027
- DOE Contract Number:
- DE-AC02-05CH11231; CA85615, CA57621, CA64786, DE-AC03-76SF00098
- Resource Type:
- Journal Article
- Journal Name:
- Clinical Cancer Research
- Additional Journal Information:
- Journal Volume: 7; Journal Issue: 11; Related Information: Journal Publication Date: 11/01/2001
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59; ADHESION; DEFECTS; FUNCTIONALS; MAMMARY GLANDS; MEMBRANES; NEOPLASMS; PHOSPHOTRANSFERASES; TYROSINE
Citation Formats
Zantek, Nicole Dodge, Walker-Daniels, Jennifer, Stewart, Jane, Hansen, Rhonda K, Robinson, Daniel, Miao, Hui, Wang, Bingcheng, Kung, Hsing-Jien, Bissell, Mina J, and Kinch, Michael S. MCF-10A-NeoST: A New Cell System for Studying Cell-ECM and Cell-Cell Interactions in Breast Cancer. United States: N. p., 2001.
Web.
Zantek, Nicole Dodge, Walker-Daniels, Jennifer, Stewart, Jane, Hansen, Rhonda K, Robinson, Daniel, Miao, Hui, Wang, Bingcheng, Kung, Hsing-Jien, Bissell, Mina J, & Kinch, Michael S. MCF-10A-NeoST: A New Cell System for Studying Cell-ECM and Cell-Cell Interactions in Breast Cancer. United States.
Zantek, Nicole Dodge, Walker-Daniels, Jennifer, Stewart, Jane, Hansen, Rhonda K, Robinson, Daniel, Miao, Hui, Wang, Bingcheng, Kung, Hsing-Jien, Bissell, Mina J, and Kinch, Michael S. 2001.
"MCF-10A-NeoST: A New Cell System for Studying Cell-ECM and Cell-Cell Interactions in Breast Cancer". United States. https://www.osti.gov/servlets/purl/1009837.
@article{osti_1009837,
title = {MCF-10A-NeoST: A New Cell System for Studying Cell-ECM and Cell-Cell Interactions in Breast Cancer},
author = {Zantek, Nicole Dodge and Walker-Daniels, Jennifer and Stewart, Jane and Hansen, Rhonda K and Robinson, Daniel and Miao, Hui and Wang, Bingcheng and Kung, Hsing-Jien and Bissell, Mina J and Kinch, Michael S},
abstractNote = {There is a continuing need for genetically matched cell systems to model cellular behaviors that are frequently observed in aggressive breast cancers. We report here the isolation and initial characterization of a spontaneously arising variant of MCF-10A cells, NeoST, which provides a new model to study cell adhesion and signal transduction in breast cancer. NeoST cells recapitulate important biological and biochemical features of metastatic breast cancer, including anchorage-independent growth, invasiveness in threedimensional reconstituted membranes, loss of E-cadherin expression, and increased tyrosine kinase activity. A comprehensive analysis of tyrosine kinase expression revealed overexpression or functional activation of the Axl, FAK, and EphA2 tyrosine kinases in transformed MCF-10A cells. MCF-10A and these new derivatives provide a genetically matched model to study defects in cell adhesion and signaling that are relevant to cellular behaviors that often typify aggressive breast cancer cells.},
doi = {},
url = {https://www.osti.gov/biblio/1009837},
journal = {Clinical Cancer Research},
number = 11,
volume = 7,
place = {United States},
year = {Wed Aug 22 00:00:00 EDT 2001},
month = {Wed Aug 22 00:00:00 EDT 2001}
}