Control of sugar transport and metabolism in Zymomonas mobilis. Final report
- Ohio State Univ., Columbus, OH (United States)
This research deals with the physiology and genetics of sugar transport and metabolic control in Zymomonas mobilis. The specific objectives of the grant as as follows: First, the complex interactions of transcriptional, post-transcriptional and translational control mechanisms on regulation of the glf operon will be investigated. Second, the structure and function of the unique glucose facilitator will be examined by a combination of in vitro and in vivo approaches, making use of the genetically reconstituted system in E. coli. Third, the possibility that physical association or indirect interactions between the glucose facilitator and glucokinase are involved in metabolic control will be analyzed. Fourth, the Z. mobilis glucose transport and phosphorylation system will be utilized to metabolically engineer recombinant E. coli with altered cell pool metabolite profiles. Work on the third and fourth objectives is complete, work on the first and second objectives is progressing nicely. Publication of this work has been admittedly slow, due primarily to a change n location of the research program from the University of Nebraska to The Ohio State University. However, it should be noted that much of the unpublished data outlined below represented completed studies, and are contained in graduate student theses which are being prepared for submission this summer. Since a full year remains in the current funding period, and the new laboratory is now up and running, we fully expect to make reasonable progress on the remaining objectives and to publish the results in a timely fashion.
- Research Organization:
- Nebraska Univ., Lincoln, NE (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG02-90ER20002
- OSTI ID:
- 100131
- Report Number(s):
- DOE/ER/20002-T2; ON: DE95017348; TRN: 95:006648
- Resource Relation:
- Other Information: PBD: [1995]
- Country of Publication:
- United States
- Language:
- English
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