Gene for the catalytic subunit of the human DNA-activated protein kinase maps to the site of the XRCC7 gene on chromosome 8
- Brookhaven National Lab., Upton, NY (United States)
- Yale Univ. School of Medicine, New Haven, CT (United States); and others
The DNA-activated serine/threonine protein kinase (DNA-PK) is composed for a large ({approximately}460 kDa) catalytic polypeptide (DNA-PK{sub cs}) and Ku, a heterodimeric DNA-binding component (p70/p80) that targets DNA-PK{sub cs} to DNA. A 41-kbp segment of the DNA-PK{sub cs} gene was isolated, and a 7902-bp segment was sequenced. The sequence contains a polymorphic Pvu II restriction enzyme site, and comparing the sequence with that of the cDNA revealed the positions of nine exons. The DNA-PK{sub cs} gene was mapped to band q11 of chromosome 8 by in situ hybridization. This location is coincident with that of XRCC7, the gene that complements the DNA double-strand break repair and V(D)J recombination defects (where V is variable, D is diversity, and J is joining) of hamster V3 and murine severe combined immunodeficient (scid) cells. 50 refs., 4 figs., 2 tabs.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 98858
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 16; Other Information: PBD: 1 Aug 1995
- Country of Publication:
- United States
- Language:
- English
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