skip to main content

SciTech ConnectSciTech Connect

Title: Chemical Profiling of the Plant Cell Wall through Raman Microspectroscopy

This paper presents a computational framework for chemical pro.ling of the plant cell wall through the Raman spectroscopy. The system enables query of known spectral signatures and clustering of spectral data based on intrinsic properties. As a result, presence and relative concentration of speci.c chemical bonds can be quanti.ed. The primary contribution of this paper is in representation of raman pro.le in terms of .uorescence background and multiscale peak detection at each grid point (voxel). Such a representation allows ef.cient spatial segmentation based on the coupling between high-level salient properties and low-level symbolic representation at each voxel. The high-level salient properties refer to preferred peaks and their attributes for the entire image. The low-level symbolic representations are based on .uorescence background, spectral peak locations, and their attributes. We present results on a corn stover tissue section that is imaged through Raman microscopy, and the results are consistent with the literature. In addition, automatic clustering indicates several distinct layers of the cell walls with different spectral signatures.
Authors:
; ; ; ; ;
Publication Date:
OSTI Identifier:
986872
Report Number(s):
LBNL-3863E
TRN: US201017%%604
DOE Contract Number:
DE-AC02-05CH11231
Resource Type:
Conference
Resource Relation:
Conference: IEEE International Symposium on Biomedical Imaging: from Nano to Macro
Research Org:
Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, CA (US)
Sponsoring Org:
Life Sciences Division
Country of Publication:
United States
Language:
English
Subject:
09; 97; AGRICULTURAL WASTES; CELL WALL; CHEMICAL BONDS; DETECTION; FLUORESCENCE; MAIZE; MICROSCOPY; PLANT CELLS; RAMAN SPECTROSCOPY Raman spectroscopy, multispectral analysis, spatial clustering