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Title: Sensitive electrochemical detection of horseradish peroxidase at disposable screen-printed carbon electrode

Abstract

A rapid, simple and sensitive electrochemical assay of horseradish peroxidase (HRP) performed on disposable screen-printed carbon electrode was developed. HRP activities were monitored by square-wave voltammetric (SWV) measuring the electroactive enzymatic product in the presence of o-aminophenol and hydrogen peroxide substrate solution. SWV analysis demonstrated a greater sensitivity and shorter analysis time than the widely used amperometric and differential-pulsed voltammetric methods. The voltammetric characteristics of substrate and enzymatic product as well as the parameters of SWV analysis were optimized. Under optimized conditions, a linear response for HRP from 0.003 - 0.1 U/mL and a detection limit of 0.002 U/mL (1.25×10-15 mol in 25 µL) were obtained with a good precision (RSD = 8%; n = 6). This rapid and sensitive HRP assay with microliters-assay volume could be readily integrated to portable devices and point-of-care (POC) diagnosis applications.

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
985032
Report Number(s):
PNNL-SA-61143
Journal ID: ISSN 1040-0397; ISSN 1521-4109; 24822; 400412000; TRN: US201016%%1725
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Electroanalysis, 20(18):2040-2046
Additional Journal Information:
Journal Volume: 20; Journal Issue: 18; Journal ID: ISSN 1040-0397
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE; ACCURACY; CARBON; DETECTION; DIAGNOSIS; ELECTRODES; HYDROGEN PEROXIDE; PEROXIDASES; SENSITIVITY; SUBSTRATES; horseradish peroxidase; electrochemical sensor; point-of-care.; Environmental Molecular Sciences Laboratory

Citation Formats

Lee, Ai Cheng, Liu, Guodong, Heng, Chew-Kiat, Tan, Swee-Nign, Lim, Tit-Meng, and Lin, Yuehe. Sensitive electrochemical detection of horseradish peroxidase at disposable screen-printed carbon electrode. United States: N. p., 2008. Web. doi:10.1002/elan.200804287.
Lee, Ai Cheng, Liu, Guodong, Heng, Chew-Kiat, Tan, Swee-Nign, Lim, Tit-Meng, & Lin, Yuehe. Sensitive electrochemical detection of horseradish peroxidase at disposable screen-printed carbon electrode. United States. https://doi.org/10.1002/elan.200804287
Lee, Ai Cheng, Liu, Guodong, Heng, Chew-Kiat, Tan, Swee-Nign, Lim, Tit-Meng, and Lin, Yuehe. 2008. "Sensitive electrochemical detection of horseradish peroxidase at disposable screen-printed carbon electrode". United States. https://doi.org/10.1002/elan.200804287.
@article{osti_985032,
title = {Sensitive electrochemical detection of horseradish peroxidase at disposable screen-printed carbon electrode},
author = {Lee, Ai Cheng and Liu, Guodong and Heng, Chew-Kiat and Tan, Swee-Nign and Lim, Tit-Meng and Lin, Yuehe},
abstractNote = {A rapid, simple and sensitive electrochemical assay of horseradish peroxidase (HRP) performed on disposable screen-printed carbon electrode was developed. HRP activities were monitored by square-wave voltammetric (SWV) measuring the electroactive enzymatic product in the presence of o-aminophenol and hydrogen peroxide substrate solution. SWV analysis demonstrated a greater sensitivity and shorter analysis time than the widely used amperometric and differential-pulsed voltammetric methods. The voltammetric characteristics of substrate and enzymatic product as well as the parameters of SWV analysis were optimized. Under optimized conditions, a linear response for HRP from 0.003 - 0.1 U/mL and a detection limit of 0.002 U/mL (1.25×10-15 mol in 25 µL) were obtained with a good precision (RSD = 8%; n = 6). This rapid and sensitive HRP assay with microliters-assay volume could be readily integrated to portable devices and point-of-care (POC) diagnosis applications.},
doi = {10.1002/elan.200804287},
url = {https://www.osti.gov/biblio/985032}, journal = {Electroanalysis, 20(18):2040-2046},
issn = {1040-0397},
number = 18,
volume = 20,
place = {United States},
year = {Wed Sep 10 00:00:00 EDT 2008},
month = {Wed Sep 10 00:00:00 EDT 2008}
}