Complete genome sequence of Rhizobium leguminosarum bv trifolii strain WSM2304, an effective microsymbiont of the South American clover Trifolium polymorphum

Journal Article · Fri Jan 01 00:00:00 EST 2010 · Standards in Genomic Sciences

DOI:https://doi.org/10.4056/sigs.44642· OSTI ID:981791

Reeve, Wayne ^[1]; O'Hara, Graham ^[1]; Chain, Patrick S. G. ^[2]; Ardley, Julie ^[1]; Brau, Lambert ^[1]; Nandesena, Kemanthi ^[1]; Tiwari, Ravi ^[1]; Malfatti, Stephanie ^[2]; Kiss, Hajnalka ^[3]; Lapidus, Alla L. ^[4]; Copeland, A ^[4]; Nolan, Matt ^[4]; Land, Miriam L ^[5]; Ivanova, N ^[4]; Mavromatis, K ^[4]; Markowitz, Victor ^[4]; Kyrpides, Nikos C ^[4]; Melino, Vanessa ^[1]; Denton, Matthew ^[6]; Yates, Ron ^[1] ; Howieson, John ^[1]

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1. Murdoch University, Perth, Australia
2. Lawrence Livermore National Laboratory (LLNL)
3. Los Alamos National Laboratory (LANL)
4. U.S. Department of Energy, Joint Genome Institute
5. ORNL
6. Department of Primary Industries, Victoria, Australia

Rhizobium leguminosarum bv trifolii is the effective nitrogen fixing microsymbiont of a diverse range of annual and perennial Trifolium (clover) species. Strain WSM2304 is an aerobic, motile, non-spore forming, Gram-negative rod, isolated from Trifolium polymorphum in Uruguay in 1998. This microsymbiont predominated in the perennial grasslands of Glencoe Research Station, in Uruguay, to competitively nodulate its host, and fix atmospheric nitrogen. Here we describe the basic features of WSM2304, together with the complete genome sequence, and annotation. This is the first completed genome sequence for a nitrogen fixing microsymbiont of a clover species from the American center of origin. We reveal that its genome size is 6,872,702 bp encoding 6,643 protein-coding genes and 62 RNA only encoding genes. This multipartite genome was found to contain 5 distinct replicons; a chromosome of size 4,537,948 bp and four circular plasmids of size 1,266,105 bp, 501,946 bp, 308,747 bp and 257,956 bp.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
• Sponsoring Organization: Work for Others (WFO)
• DOE Contract Number: DE-AC05-00OR22725
• OSTI ID: 981791
• Journal Information: Standards in Genomic Sciences, Vol. 2, Issue 1; ISSN 1944-3277
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE
CHROMOSOMES
CLOVER
GENES
NITROGEN
ORIGIN
PLASMIDS
RANGELANDS
REPLICONS
RHIZOBIUM
RNA
STRAINS
microsymbiont
non-pathogenic
aerobic
Gram-negative rod
root-nodule bacteria
nitrogen fixation
Alphaproteobacteria