Preliminary X-ray Data Analysis of Crystalline Hibiscus Chlorotic Ringspot Virus
Hibiscus chlorotic ringspot virus (HCRSV) is a positive-sense monopartite single-stranded RNA virus that belongs to the Carmovirus genus of the Tombusviridae family, which includes carnation mottle virus (CarMV). The HCRSV virion has a 30 nm diameter icosahedral capsid with T = 3 quasi-symmetry containing 180 copies of a 38 kDa coat protein (CP) and encapsidates a full-length 3.9 kb genomic RNA. Authentic virus was harvested from infected host kenaf leaves and was purified by saturated ammonium sulfate precipitation, sucrose density-gradient centrifugation and anion-exchange chromatography. Virus crystals were grown in multiple conditions; one of the crystals diffracted to 3Synchrotron .2 A resolution and allowed the collection of a partial data set. The crystal belonged to space group R32, with unit-cell parameters a = b = 336.4, c = 798.5 . Packing considerations and rotation-function analysis determined that there were three particles per unit cell, all of which have the same orientation and fixed positions, and resulted in tenfold noncrystallography symmetry for real-space averaging. The crystals used for the structure determination of southern bean mosaic virus (SBMV) have nearly identical characteristics. Together, these findings will greatly aid the high-resolution structure determination of HCRSV.
- Research Organization:
- Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source
- Sponsoring Organization:
- Doe - Office Of Science
- DOE Contract Number:
- DE-AC02-98CH10886
- OSTI ID:
- 980399
- Report Number(s):
- BNL-93317-2010-JA; TRN: US201015%%1784
- Journal Information:
- Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 65, Issue 6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE
AMMONIUM SULFATES
BEANS
CARNATIONS
CENTRIFUGATION
CHROMATOGRAPHY
CRYSTALS
DATA ANALYSIS
HOST
LEAVES
ORIENTATION
PARTICLES
PRECIPITATION
PROTEINS
RESOLUTION
RNA
SACCHAROSE
SPACE GROUPS
STOWING
SYMMETRY
UNITS
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