Comparison of the complete genome sequences of Pseudomonas syringae pv. syringae B728a and pv. tomato DC3000

Journal Article · Sat Jan 01 00:00:00 EST 2005 · Proceedings of the National Academy of Sciences

DOI:https://doi.org/10.1073/pnas.0504930102· OSTI ID:978723

Feil, Helene ^[1]; Feil, William ^[1]; Chain, Patrick S. G. ^[2]; Larimer, Frank W ^[3]; DiBartolo, Genevieve ^[4]; Copeland, A ^[4]; Lykidis, A ^[4]; Trong, Stephen ^[4]; Nolan, Matt ^[4]; Goltsman, Eugene ^[4]; Thiel, James ^[4]; Malfatti, Stephanie ^[2]; Loper, Joyce E ^[5]; Detter, J C ^[4]; Lapidus, Alla L ^[4]; Land, Miriam L ^[3]; Richardson, P M ^[4]; Kyrpides, Nikos C ^[4]; Ivanova, N ^[4]; Lindow, Steven E ^[1]

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1. University of California, Berkeley
2. Lawrence Livermore National Laboratory (LLNL)
3. ORNL
4. U.S. Department of Energy, Joint Genome Institute
5. U.S. Department of Agriculture, Agricultural Research Service

The complete genomic sequence of Pseudomonas syringae pv. syringae B728a (Pss B728a) has been determined and is compared with that of A syringae pv. tomato DC3000 (Pst DC3000). The two pathovars of this economically important species of plant pathogenic bacteria differ in host range and other interactions with plants, with Pss having a more pronounced epiphytic stage of growth and higher abiotic stress tolerance and Pst DC3000 having a more pronounced apoplastic growth habitat. The Pss B728a genome (6.1 Mb) contains a circular chromosome and no plasmid, whereas the Pst DC3000 genome is 6.5 mbp in size, composed of a circular chromosome and two plasmids. Although a high degree of similarity exists between the two sequenced Pseudomonads, 976 protein-encoding genes are unique to Pss B728a when compared with Pst DC3000, including large genomic islands likely to contribute to virulence and host specificity. Over 375 repetitive extragenic palindromic sequences unique to Pss B728a when compared with Pst DC3000 are widely distributed throughout the chromosome except in 14 genomic islands, which generally had lower GC content than the genome as a whole. Content of the genomic islands varies, with one containing a prophage and another the plasmid pKLC102 of Pseudomonas aeruginosa PAO1. Among the 976 genes of Pss B728a with no counterpart in Pst DC3000 are those encoding for syringopeptin, syringomycin, indole acetic acid biosynthesis, arginine degradation, and production of ice nuclei. The genomic comparison suggests that several unique genes for Pss B728a such as ectoine synthase, DNA repair, and antibiotic production may contribute to the epiphytic fitness and stress tolerance of this organism.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
• Sponsoring Organization: USDOE Office of Science (SC)
• DOE Contract Number: DE-AC05-00OR22725
• OSTI ID: 978723
• Journal Information: Proceedings of the National Academy of Sciences, Vol. 102, Issue 31
• Country of Publication: United States
• Language: English