A novel fluorescence imaging technique combining deconvolution microscopy and spectral analysis for quantitative detection of opportunistic pathogens
Journal Article
·
· Journal of Microbiological Methods
- Florida Gulf Coast University
- University of Tennessee, Knoxville (UTK)
- ORNL
- University of Central Florida
A novel fluorescence imaging technique based on deconvolution microscopy and spectral analysis is presented here as an alternative to confocal laser scanning microscopy. It allowed rapid, specific and simultaneous identification of five major opportunistic pathogens, relevant for public health, in suspension and provided quantitative results.
- Research Organization:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Sponsoring Organization:
- USDOE Laboratory Directed Research and Development (LDRD) Program
- DOE Contract Number:
- DE-AC05-00OR22725
- OSTI ID:
- 963376
- Journal Information:
- Journal of Microbiological Methods, Vol. 67, Issue 3; ISSN 0167-7012
- Country of Publication:
- United States
- Language:
- English
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