Gene and protein expression profiles of Shewanella oneidensis during anaerobic growth with different electron acceptors.

Journal Article · Tue Jan 01 00:00:00 EST 2002 · Omics

DOI:https://doi.org/10.1089/15362310252780834· OSTI ID:961430

Beliaev, A S; Thompson, D K; Khare, T; Lim, H; Brandt, C C; Li, G; Murray, A E; Heidelberg, J F; Giometti, C S; Nealson, K H; Tiedje, J M; Zhou, J; ORNL,

Changes in mRNA and protein expression profiles of Shewanella oneidenesis MR-1 during switch from aerobic to fumarate-, Fe(III)-, or nitrate-reducing conditions were examined using DNA microarrays and two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). In response to changes in growth conditions, 121 of the 691 arrayed genes displayed at least a two-fold difference in transcript abundance as determined by microarray analysis. Genes involved in aerobic respiration encoding cytochrome c and d oxidases and TCA cycle enzymes were repressed under anaerobic conditions. Genes induced during anaerobic respiration included those involved in cofactor biosynthesis and assembly (moaACE, ccmHF, nosD, cysG), substrate transport (cysUP, cysTWA, dcuB), and anaerobic energy metabolism (dmsAB, psrC, pshA, hyaABC, hydA). Transcription of genes encoding a periplasmic nitrate reductase (napBHGA), cytochrome c{sub 552}, and prismane was elevated 8- to 56-fold in response to the presence of nitrate, while cymA, ifcA, and frdA were specifically induced three- to eightfold under fumarate-reducing conditions. The mRNA levels for two oxidoreductase-like genes of unknown function and several cell envelope genes involved in multidrug resistance increased two- to fivefold specifically under Fe(III)-reducing conditions. Analysis of protein expression profiles under aerobic and anaerobic conditions revealed 14 protein spots that showed significant differences in abundance on 2-D gels. Protein identification by mass spectrometry indicated that the expression of prismane, dihydrolipoamide succinyltransferase, and alcaligin siderophore biosynthesis protein correlated with the microarray data.

• Research Organization: Argonne National Lab. (ANL), Argonne, IL (United States)
• Sponsoring Organization: USDOE Office of Science (SC)
• DOE Contract Number: DE-AC02-06CH11357
• OSTI ID: 961430
• Report Number(s): ANL/BIO/JA-47688; TRN: US201012%%1493
• Journal Information: Omics, Vol. 6, Issue 1 ; 2002
• Country of Publication: United States
• Language: ENGLISH

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE
ABUNDANCE
ANAEROBIC CONDITIONS
BINDING ENERGY
BIOSYNTHESIS
CYTOCHROMES
DNA
ELECTRONS
ELECTROPHORESIS
ENZYMES
GELS
GENES
GROWTH
IRON
MASS SPECTROSCOPY
MESSENGER-RNA
METABOLISM
NITRATES
OXIDASES
OXIDOREDUCTASES
PROTEINS
RESPIRATION
REDUCTION
SUBSTRATES
TRANSCRIPTION
TRANSPORT
VALENCE