Heterologous Expression of Glycosyl Hydrolases in planta: A New Departure for Biofuels

Taylor II, Larry E.; Dai, Ziyu; Decker, Steven R.; Brunecky, Roman; Adney, William S.; Ding, Shi-You; Himmel, Michael E.

doi:10.1016/j.tibtech.2008.05.002

Title: Heterologous Expression of Glycosyl Hydrolases in planta: A New Departure for Biofuels

Journal Article · Tue Jan 01 00:00:00 EST 2008 · Trends in Biotechnology

DOI:https://doi.org/10.1016/j.tibtech.2008.05.002· OSTI ID:940654

Taylor II, Larry E.; Dai, Ziyu;

; Brunecky, Roman; Adney, William S.; Ding, Shi-You; Himmel, Michael E.

The concept of expressing non-plant glycosyl hydrolase genes in plant tissue is nearly two decades old, yet relatively little work in this field has been reported. However, resurgent interest in technologies aimed at enabling processes that convert biomass to sugars and fuels has turned attention toward this intuitive solution. There are several challenges facing researchers in this field, including the development of better and more specifically targeted delivery systems for hydrolytic genes, the successful folding and post-translational modification of heterologous proteins and the development of cost-effective process strategies utilizing these transformed plants. The integration of these concepts, from the improvement of biomass production and conversion characteristics to the heterologous production of glycosyl hydrolases in a high yielding bioenergy crop, holds considerable promise for improving the lignocellulosic conversion of biomass to ethanol and subsequently to fuels.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of the Biomass Program

DOE Contract Number:: AC36-08GO28308

OSTI ID:: 940654

Report Number(s):: NREL/JA-270-43082; TRBIDM; MainId:47504; UUID:e388c796-f0d4-e411-b769-d89d67132a6d; MainAdminId:29596; TRN: US200824%%149

Journal Information:: Trends in Biotechnology, Vol. 26, Issue 8; ISSN 0167-7799

Country of Publication:: United States

Language:: English

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