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Title: Detection of in-situ derivatized peptides in microbial biofilms by laser desorption 7.87 eV postionizaton mass spectrometry.

Abstract

A novel analytical method based on laser desorption postionization mass spectrometry (LDPI-MS) was developed to investigate the competence and sporulation factor-a pentapeptide of amino acid sequence ERGMT-within intact Bacillus subtilis biofilms. Derivatization of the neat ERGMT peptide with quinoline- and anthracene-based tags was separately used to lower the peptide ionization potential and permit direct ionization by 7.87-eV vacuum ultraviolet radiation. The techniques of mass shifting and selective ionization of the derivatized peptide were combined here to permit detection of ERGMT peptide within intact biofilms by LDPI-MS, without any prior extraction or chromatographic separation. Finally, imaging MS specific to the derivatized peptide was demonstrated on an intact biofilm using LDPI-MS. The presence of ERGMT in the biofilms was verified by bulk extraction/LC-MS. However, MALDI imaging MS analyses were unable to detect ERGMT within intact biofilms.

Authors:
; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
939554
Report Number(s):
ANL/BIO/JA-57364
Journal ID: ISSN 0003-2700; ANCHAM; TRN: US200823%%265
DOE Contract Number:  
DE-AC02-06CH11357
Resource Type:
Journal Article
Journal Name:
Anal. Chem.
Additional Journal Information:
Journal Volume: 79; Journal Issue: 2 ; 2007; Journal ID: ISSN 0003-2700
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; AMINO ACID SEQUENCE; BACILLUS SUBTILIS; DERIVATIZATION; DESORPTION; DETECTION; FAR ULTRAVIOLET RADIATION; IONIZATION; IONIZATION POTENTIAL; LASERS; MASS SPECTROSCOPY; PEPTIDES

Citation Formats

Edirisinghe, P D, Moore, J F, Skinner-Nemec, K A, Lindberg, C, Giometti, C S, Veryovkin, I V, Hunt, J E, Pellin, M J, Hanley, L, Biosciences Division, Univ. of Illinois at Chicago, and MassThink,. Detection of in-situ derivatized peptides in microbial biofilms by laser desorption 7.87 eV postionizaton mass spectrometry.. United States: N. p., 2007. Web. doi:10.1021/ac0615605.
Edirisinghe, P D, Moore, J F, Skinner-Nemec, K A, Lindberg, C, Giometti, C S, Veryovkin, I V, Hunt, J E, Pellin, M J, Hanley, L, Biosciences Division, Univ. of Illinois at Chicago, & MassThink,. Detection of in-situ derivatized peptides in microbial biofilms by laser desorption 7.87 eV postionizaton mass spectrometry.. United States. https://doi.org/10.1021/ac0615605
Edirisinghe, P D, Moore, J F, Skinner-Nemec, K A, Lindberg, C, Giometti, C S, Veryovkin, I V, Hunt, J E, Pellin, M J, Hanley, L, Biosciences Division, Univ. of Illinois at Chicago, and MassThink,. 2007. "Detection of in-situ derivatized peptides in microbial biofilms by laser desorption 7.87 eV postionizaton mass spectrometry.". United States. https://doi.org/10.1021/ac0615605.
@article{osti_939554,
title = {Detection of in-situ derivatized peptides in microbial biofilms by laser desorption 7.87 eV postionizaton mass spectrometry.},
author = {Edirisinghe, P D and Moore, J F and Skinner-Nemec, K A and Lindberg, C and Giometti, C S and Veryovkin, I V and Hunt, J E and Pellin, M J and Hanley, L and Biosciences Division and Univ. of Illinois at Chicago and MassThink,},
abstractNote = {A novel analytical method based on laser desorption postionization mass spectrometry (LDPI-MS) was developed to investigate the competence and sporulation factor-a pentapeptide of amino acid sequence ERGMT-within intact Bacillus subtilis biofilms. Derivatization of the neat ERGMT peptide with quinoline- and anthracene-based tags was separately used to lower the peptide ionization potential and permit direct ionization by 7.87-eV vacuum ultraviolet radiation. The techniques of mass shifting and selective ionization of the derivatized peptide were combined here to permit detection of ERGMT peptide within intact biofilms by LDPI-MS, without any prior extraction or chromatographic separation. Finally, imaging MS specific to the derivatized peptide was demonstrated on an intact biofilm using LDPI-MS. The presence of ERGMT in the biofilms was verified by bulk extraction/LC-MS. However, MALDI imaging MS analyses were unable to detect ERGMT within intact biofilms.},
doi = {10.1021/ac0615605},
url = {https://www.osti.gov/biblio/939554}, journal = {Anal. Chem.},
issn = {0003-2700},
number = 2 ; 2007,
volume = 79,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 2007},
month = {Mon Jan 01 00:00:00 EST 2007}
}