The Potential and Challenges of Nanopore Sequencing

Journal Article · Wed Oct 01 00:00:00 EDT 2008 · Nature Biotechnology

DOI:https://doi.org/10.1038/nbt.1495· OSTI ID:939411

Branton, Daniel ^[1]; Deamer, D W ^[2]; Marziali, A ^[3]; Bayley, H ^[4]; Benner, S A ^[5]; Butler, Thomas ^[6]; Di Ventra, Massimiliano ^[7]; Garaj, S ^[1]; Hibbs, Andrew ^[8]; Huang, Xiaohua ^[7]; Jovanovich, Stevan B ^[9]; Krstic, Predrag S ^[10]; Lindsay, Stuart ^[11]; Ling, Xinsheng Sean ^[12]; Mastrangelo, Carlos H ^[13]; Meller, Amit ^[1]; Oliver, John S ^[14]; Pershin, Yuriy V ^[7]; Ramsey, Dr John Michael ^[15]; Riehn, Robert ^[16] ; Soni, Gautam ^[17]; Tabard-Cossa, Vincent ^[3]; Wanuunu, Meni ^[17]; Wiggin, Matthew ^[3]; Schloss, Jeffrey A ^[10]

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1. Harvard University
2. University of California, Santa Cruz
3. University of British Columbia, Vancouver
4. University of Oxford
5. Foundation for Applied Molecular Evolution, Gainesville, FL
6. University of Washington, Seattle
7. University of California, San Diego
8. Electronic BioSciences, San Diego, CA
9. Microchip BioTechnologies Inc., Dublin, CA
10. ORNL
11. Arizona State University
12. Brown University
13. Case Western Reserve University
14. NABsys, Inc., Providence, RI
15. University of North Carolina
16. North Carolina State University
17. Boston University

A nanopore-based device provides single-molecule detection and analytical capabilities that are achieved by electrophoretically driving molecules in solution through a nan-scale pore. The nanopore provides a highly confined space within which single nucleic acid polymers can be analyzed at high throughput by one of a variety of means, and the perfect processivity that can be enforced in a narrow pore ensures that the native order of the nucleobases in a polynucleotide is reflected in the sequence of signals that is detected. Kilobase length polymers (single-stranded genomic DNA or RNA) or small molecules (e.g., nucleosides) can be identified and characterized without amplification or labeling, a unique analytical capability that makes inexpensive, rapid DNA sequencing a possibility. Further research and development to overcome current challenges to nanopore identification of each successive nucleotide in a DNA strand offers the prospect of 'third generation' instruments that will sequence a diploid mammalian genome for ~$1,000 in ~24 h.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
• Sponsoring Organization: USDOE Office of Science (SC)
• DOE Contract Number: DE-AC05-00OR22725
• OSTI ID: 939411
• Journal Information: Nature Biotechnology, Vol. 26, Issue 10; ISSN 1087-0156
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AMPLIFICATION
DETECTION
DNA
DNA SEQUENCING
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
POLYMERS
RNA