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Title: EPICS controlled sample mounting robots at the GM/CA CAT.

Abstract

GM/CA CAT at Sector 23 of the advanced photon source (APS) is an NIH funded facility for crystallographic structure determination of biological macromolecules by X-ray diffraction [R.F. Fischetti, et al., GM/CA canted undulator beamlines for protein crystallography, Acta Crystallogr. A 61 (2005) C139]. The facility consists of three beamlines; two based on canted undulators and one on a bending magnet. The scientific and technical goals of the CAT emphasize streamlined, efficient throughput for a variety of sample types, sizes and qualities, representing the cutting edge of structural biology research. For this purpose all three beamlines are equipped with the ALS-style robots [C.W.Cork, et al. Status of the BCSB automated sample mounting and alignment system for macromolecular crystallography at the Advanced Light Source, SRI-2003, San-Francisco, CA, USA, August 25-29, 2003] for an automated mounting of cryo-protected macromolecular crystals. This report summarizes software and technical solutions implemented with the first of the three operational robots at beamline 23-ID-B. The automounter's Dewar can hold up to 72 or 96 samples residing in six Rigaku ACTOR magazines or ALS-style pucks, respectively. Mounting of a crystal takes approximately 2 s, during which time the temperature of the crystal is maintained near that of liquid nitrogen.

Authors:
; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
National Cancer Inst.; National Inst.of General Medical Science
OSTI Identifier:
939330
Report Number(s):
ANL/BIO/JA-60440
Journal ID: ISSN 0168-9002; NIMAER; TRN: US0806755
DOE Contract Number:  
DE-AC02-06CH11357
Resource Type:
Journal Article
Journal Name:
Nucl. Instrum. Methods Phys. Res. A
Additional Journal Information:
Journal Volume: 582; Journal Issue: 1 ; Nov. 11, 2007; Journal ID: ISSN 0168-9002
Country of Publication:
United States
Language:
ENGLISH
Subject:
43 PARTICLE ACCELERATORS; ADVANCED LIGHT SOURCE; ADVANCED PHOTON SOURCE; ALIGNMENT; BENDING; BIOLOGY; CRYSTALLOGRAPHY; DEWARS; NITROGEN; PROTEINS; ROBOTS; WIGGLER MAGNETS; X-RAY DIFFRACTION

Citation Formats

Makarov, O A, Benn, R, Corcoran, S, Devarapalli, S, Fischetti, R, Hilgart, M, Smith, W W, Stepanov, S, Xu, S, and Biosciences Division. EPICS controlled sample mounting robots at the GM/CA CAT.. United States: N. p., 2007. Web. doi:10.1016/j.nima.2007.08.096.
Makarov, O A, Benn, R, Corcoran, S, Devarapalli, S, Fischetti, R, Hilgart, M, Smith, W W, Stepanov, S, Xu, S, & Biosciences Division. EPICS controlled sample mounting robots at the GM/CA CAT.. United States. https://doi.org/10.1016/j.nima.2007.08.096
Makarov, O A, Benn, R, Corcoran, S, Devarapalli, S, Fischetti, R, Hilgart, M, Smith, W W, Stepanov, S, Xu, S, and Biosciences Division. 2007. "EPICS controlled sample mounting robots at the GM/CA CAT.". United States. https://doi.org/10.1016/j.nima.2007.08.096.
@article{osti_939330,
title = {EPICS controlled sample mounting robots at the GM/CA CAT.},
author = {Makarov, O A and Benn, R and Corcoran, S and Devarapalli, S and Fischetti, R and Hilgart, M and Smith, W W and Stepanov, S and Xu, S and Biosciences Division},
abstractNote = {GM/CA CAT at Sector 23 of the advanced photon source (APS) is an NIH funded facility for crystallographic structure determination of biological macromolecules by X-ray diffraction [R.F. Fischetti, et al., GM/CA canted undulator beamlines for protein crystallography, Acta Crystallogr. A 61 (2005) C139]. The facility consists of three beamlines; two based on canted undulators and one on a bending magnet. The scientific and technical goals of the CAT emphasize streamlined, efficient throughput for a variety of sample types, sizes and qualities, representing the cutting edge of structural biology research. For this purpose all three beamlines are equipped with the ALS-style robots [C.W.Cork, et al. Status of the BCSB automated sample mounting and alignment system for macromolecular crystallography at the Advanced Light Source, SRI-2003, San-Francisco, CA, USA, August 25-29, 2003] for an automated mounting of cryo-protected macromolecular crystals. This report summarizes software and technical solutions implemented with the first of the three operational robots at beamline 23-ID-B. The automounter's Dewar can hold up to 72 or 96 samples residing in six Rigaku ACTOR magazines or ALS-style pucks, respectively. Mounting of a crystal takes approximately 2 s, during which time the temperature of the crystal is maintained near that of liquid nitrogen.},
doi = {10.1016/j.nima.2007.08.096},
url = {https://www.osti.gov/biblio/939330}, journal = {Nucl. Instrum. Methods Phys. Res. A},
issn = {0168-9002},
number = 1 ; Nov. 11, 2007,
volume = 582,
place = {United States},
year = {Sun Nov 11 00:00:00 EST 2007},
month = {Sun Nov 11 00:00:00 EST 2007}
}