Community proteogenomics highlights microbial strain-variant protein expression within activated sludge performing enhanced biological phosphorus removal.

Journal Article · Tue Jan 01 00:00:00 EST 2008 · The ISME Journal: Multidisciplinary Journal of Microbial Ecology

DOI:https://doi.org/10.1038/ismej.2008.38· OSTI ID:935738

Wilmes, P ^[1]; Andersson, Anders F. ^[1]; Lefsrud, Mark G ^[2]; Wexler, Margaret ^[3]; Shah, Manesh B ^[4]; Zhang, B ^[5]; Bond, P. L. ^[6]; Verberkmoes, Nathan C ^[4]; Banfield, Jillian F. ^[1]

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1. University of California, Berkeley
2. McGill University, Montreal, Quebec
3. University of East Anglia, Norwich, United Kingdom
4. ORNL
5. Vanderbilt University
6. University of Queensland, The, Brisbane, Queensland, Australia

Enhanced biological phosphorus removal (EBPR) selects for polyphosphate accumulating organisms to achieve phosphate removal from wastewater. We used highresolution community proteomics to identify key metabolic pathways in "Candidatus Accumulibacter phosphatis"-mediated EBPR and to evaluate the contributions of co- 5 existing strains within the dominant population. Results highlight the importance of denitrification, fatty acid cycling and the glyoxylate bypass in EBPR. Despite overall strong similarity in protein profiles under anaerobic and aerobic conditions, fatty acid degradation proteins were more abundant during the anaerobic phase. By comprehensive genome-wide alignment of orthologous proteins, we uncovered strong 10 functional partitioning for enzyme variants involved in both core-metabolism and EBPR-specific pathways among the dominant strains. These findings emphasize the importance of genetic diversity in maintaining the stable performance of EBPR systems and demonstrate the power of integrated cultivation-independent genomics and proteomics for analysis of complex biotechnological systems.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
• Sponsoring Organization: USDOE Office of Science (SC)
• DOE Contract Number: DE-AC05-00OR22725
• OSTI ID: 935738
• Journal Information: The ISME Journal: Multidisciplinary Journal of Microbial Ecology, Vol. 2, Issue 8
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AEROBIC CONDITIONS
ALIGNMENT
BIOLOGICAL PATHWAYS
CARBOXYLIC ACIDS
DENITRIFICATION
ENZYMES
FUNCTIONALS
GENETICS
PERFORMANCE
PHOSPHATES
PHOSPHORUS
PROTEINS
REMOVAL
SLUDGES
STRAINS