GeoChip: A comprehensive microarray for investigatingbiogeochemical, ecological, and environmental processes
Due to their vast diversity and as-yet uncultivated status,detection, characterization and quantification of microorganisms innatural settings are very challenging, and linking microbial diversity toecosystem processes and functions is even more difficult.Microarray-based genomic technology for detecting functional genes andprocesses has a great promise of overcoming such obstacles. Here, a novelcomprehensive microarray, termed GeoChip, has been developed, containing24,243 oligonucleotide (50mer) probes and covering>10,000 genes in>150 functional groups involved in nitrogen, carbon, sulfur andphosphorus cycling, metal reduction and resistance, and organiccontaminant degradation. The developed GeoChip was successfully used fortracking the dynamics of metal-reducing bacteria and associatedcommunities for an in situ bioremediation study, which is the first timeto demonstrate that uranium can be bioremediated to the concentrationsbelow the USA EPA maximum contaminant level (MCL) for drinking water.This is the first comprehensive microarray available for studyingbiogeochemical processes and functional activities of microbialcommunities important to human health, agriculture, energy, globalclimate change, ecosystem management, and environmental cleanup andrestoration. It is particularly useful for providing direct linkages ofmicrobial genes/populations to ecosystem processes andfunctions.
- Research Organization:
- COLLABORATION - University of Oklahoma, Norman,OK
- DOE Contract Number:
- DE-AC02-05CH11231
- OSTI ID:
- 932980
- Report Number(s):
- LBNL-63453; R&D Project: VGTLJK; TRN: US200814%%189
- Journal Information:
- The ISME Journal, Vol. 1, Issue 1; Related Information: Journal Publication Date: 2007
- Country of Publication:
- United States
- Language:
- English
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