Using Barium Ions for Heavy-Atom Derivatization and Phasing of Xylanse ll from Trichoderma longibrachiatum

Journal Article · Mon Jan 01 00:00:00 EST 2007 · Acta Crystallographica Section D: Biological Crystallography

DOI:https://doi.org/10.1107/S090744490703418X· OSTI ID:929953

Moiseeva, N; Allaire, M

This paper describes the use of barium chloride to produce a heavy-atom derivative of xylanase II crystals from Trichoderma longibrachiatum, which was obtained either by cocrystallization or soaking. SAD phasing led to interpretable electron-density maps that allowed unambiguous chain tracing. In the best case, with a data set collected at 9.5 keV, 88% of the residues were built, with 83% of the side chains assigned. The barium ions are found to mainly interact with main-chain carbonyl groups and water molecules. It is suggested that barium ions could also be used as a potential anomalous scatterer in the quick cryosoaking procedure for phasing.

• Research Organization: Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source
• Sponsoring Organization: Doe - Office Of Science
• DOE Contract Number: DE-AC02-98CH10886
• OSTI ID: 929953
• Report Number(s): BNL-80548-2008-JA; TRN: US0806673
• Journal Information: Acta Crystallographica Section D: Biological Crystallography, Vol. 63
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
74 ATOMIC AND MOLECULAR PHYSICS
BARIUM CHLORIDES
BARIUM IONS
CARBONYLS
CRYSTALS
DATA
DERIVATIZATION
ELECTRON DENSITY
MAPS
MOLECULES
POTENTIALS
RESIDUES
TRICHODERMA
WATER
XYLANASE
national synchrotron light source