Antioxidant capacity of cultured mammalian cells estimated by ESRmethod.
In the present study, the antioxidant capacity againsthydrogen peroxide (H2O2), one of the stress-inducing agents, wasinvestigated in two distinct cell lines: L-41 (human epithelial-likecells) and HLF (human diploid lung fibroblasts), which differ in tissueorigin, life span in culture, proliferate activity, and special enzymesystem activity. The cell antioxidant capacity against H2O2 was estimatedby the electron spin resonance (ESR) spin-trapping technique in theFenton reaction system via Fe+2 ion action with H2O2 resulting inhydroxyl radical generation. The effects of catalase inhibitors, such assodium azide and 3-amino-1,2,4-triazole, on the antioxidant capacity ofcells were tested. Based on our observation, it can be concluded that thedefensive capacity of cells against H2O2 depends on the ratio betweencatalase/GPx/SOD and H2O2, especially at high-stress situations, and theintracellular balance of these enzymes are more important than theinfluence of the single component.
- Research Organization:
- COLLABORATION - Georgian Academy ofSciences
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- DE-AC02-05CH11231
- OSTI ID:
- 926581
- Report Number(s):
- LBNL-60924; R&D Project: G430; BnR: KP1102010
- Journal Information:
- Scientific World Journal, Vol. 4; Related Information: Journal Publication Date: 29 June 2004
- Country of Publication:
- United States
- Language:
- English
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