Bismuth Dimercaptopropanol (BisBAL) Inhibits the Expression of Extracellular Polysaccharides and Proteins by Brevundimonas diminuta: Implications for Membrane Microfiltration

Journal Article · Fri Feb 15 00:00:00 EST 2008 · Biotechnology and Bioengineering, 99(3):634-643

DOI:https://doi.org/10.1002/bit.21615· OSTI ID:924041

Badireddy, Appala R; Chellam, Shankararaman; Yanina, Svetlana; Gassman, Paul L; Rosso, Kevin M

A 2:1 molar ratio preparation of bismuth with a lipophilic dithiol (3-dimercapto-1-propanol, BAL)significantly reduced extracellular polymeric substances (EPS) expression by Brevundimonas diminuta in suspended cultures at levels just below the minimum inhibitory concentration (MIC). Total polysaccharides and proteins secreted by B. diminuta decreased by approximately 95% over a 5-day period when exposed to the bismuth-BAL chelate (BisBAL) at near MIC (12 μM). Fourier-transform infrared spectroscopy (FTIR) suggested that a possible mechanism of biofilm disruption by BisBAL is the inhibition of carbohydrate Oacetylation. FTIR also revealed extensive homology between EPS samples with and without BisBAL treatment, with proteins, polysaccharides, and peptides varying predominantly only in the amount expressed. EPS secretion decreased following BisBAL treatment as verified by atomic force microscopy and scanning electron microscopy. Without BisBAL treatment, a slime-like EPS matrix secreted by B. diminuta resulted in biofouling and inefficient hydrodynamic backwashing of microfiltration membranes.

• Research Organization: Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 924041
• Report Number(s): PNNL-SA-56407; BIBIAU; 17096; KP1704020; TRN: US200805%%97
• Journal Information: Biotechnology and Bioengineering, 99(3):634-643, Vol. 99, Issue 3; ISSN 0006-3592
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ATOMIC FORCE MICROSCOPY
BIOLOGICAL FOULING
BISMUTH
CARBOHYDRATES
CHELATES
DIMERCAPROL
DITHIOLS
HYDRODYNAMICS
MEMBRANES
PEPTIDES
POLYSACCHARIDES
PROTEINS
SCANNING ELECTRON MICROSCOPY
SECRETION
SPECTROSCOPY
microfiltration
extracellular polymeric substances
biofilm
biofouling
ultrafiltration
bismuth
Environmental Molecular Sciences Laboratory