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Title: Fully automated four-column capillary LC-MS system for maximizing throughput in proteomic analyses

Abstract

We describe a 4-column, high-pressure capillary liquid chromatography (LC) system for robust, high-throughput LC-MS(/MS) analyses. This system performs multiple LC separations in parallel, but staggers each of them such that the data-rich region of each separation is sampled sequentially. By allowing nearly continuous data acquisition, this design maximizes the use of the mass spectrometer. Each analytical column is connected to a corresponding ESI emitter in order to avoid the use of post-column switching and associated dead volume issues. Encoding translation stages are employed to sequentially position the emitters at the MS inlet. The high reproducibility of this system is demonstrated using consecutive analyses of global tryptic digest of the microbe Shewanella oneidensis.

Authors:
; ; ; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
921809
Report Number(s):
PNNL-SA-56532
400412000; TRN: US200802%%913
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Analytical Chemistry, 80(1):294-302
Additional Journal Information:
Journal Volume: 80; Journal Issue: 1
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; LIQUID COLUMN CHROMATOGRAPHY; DESIGN; MASS SPECTROMETERS; CAPILLARY FLOW; AUTOMATION; DATA ACQUISITION

Citation Formats

Livesay, Eric A, Tang, Keqi, Taylor, Beverley K, Buschbach, Michael A, Hopkins, Derek F, Lamarche, Brian L, Zhao, Rui, Shen, Yufeng, Orton, Daniel J, Moore, Ronald J, Kelly, Ryan T, Udseth, Harold R, and Smith, Richard D. Fully automated four-column capillary LC-MS system for maximizing throughput in proteomic analyses. United States: N. p., 2008. Web. doi:10.1021/ac701727r.
Livesay, Eric A, Tang, Keqi, Taylor, Beverley K, Buschbach, Michael A, Hopkins, Derek F, Lamarche, Brian L, Zhao, Rui, Shen, Yufeng, Orton, Daniel J, Moore, Ronald J, Kelly, Ryan T, Udseth, Harold R, & Smith, Richard D. Fully automated four-column capillary LC-MS system for maximizing throughput in proteomic analyses. United States. https://doi.org/10.1021/ac701727r
Livesay, Eric A, Tang, Keqi, Taylor, Beverley K, Buschbach, Michael A, Hopkins, Derek F, Lamarche, Brian L, Zhao, Rui, Shen, Yufeng, Orton, Daniel J, Moore, Ronald J, Kelly, Ryan T, Udseth, Harold R, and Smith, Richard D. 2008. "Fully automated four-column capillary LC-MS system for maximizing throughput in proteomic analyses". United States. https://doi.org/10.1021/ac701727r.
@article{osti_921809,
title = {Fully automated four-column capillary LC-MS system for maximizing throughput in proteomic analyses},
author = {Livesay, Eric A and Tang, Keqi and Taylor, Beverley K and Buschbach, Michael A and Hopkins, Derek F and Lamarche, Brian L and Zhao, Rui and Shen, Yufeng and Orton, Daniel J and Moore, Ronald J and Kelly, Ryan T and Udseth, Harold R and Smith, Richard D},
abstractNote = {We describe a 4-column, high-pressure capillary liquid chromatography (LC) system for robust, high-throughput LC-MS(/MS) analyses. This system performs multiple LC separations in parallel, but staggers each of them such that the data-rich region of each separation is sampled sequentially. By allowing nearly continuous data acquisition, this design maximizes the use of the mass spectrometer. Each analytical column is connected to a corresponding ESI emitter in order to avoid the use of post-column switching and associated dead volume issues. Encoding translation stages are employed to sequentially position the emitters at the MS inlet. The high reproducibility of this system is demonstrated using consecutive analyses of global tryptic digest of the microbe Shewanella oneidensis.},
doi = {10.1021/ac701727r},
url = {https://www.osti.gov/biblio/921809}, journal = {Analytical Chemistry, 80(1):294-302},
number = 1,
volume = 80,
place = {United States},
year = {Tue Jan 01 00:00:00 EST 2008},
month = {Tue Jan 01 00:00:00 EST 2008}
}