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Title: Catching RNA Polymerase in the act of Binding: Intermediates in Transcription Illuminated by Synchrotron Footprinting

Abstract

The article by Sclavi et al. in this issue of PNAS addresses 'initiation, ' the first step in transcription. Gene transcription is catalyzed in cells by large multisubunit proteins called RNA polymerases (RNAP). The eubacteria holoenzyme of RNAP is composed of five core subunits ({alpha}, {alpha}2, {beta}, {beta}', and {omega}) that contain the amino acid residues required for the enzyme's catalytic activity. A sixth subunit ({sigma}) guides RNAP to specific sequences on the genomic DNA (promoters) that mark the beginning of a gene or group of genes.

Authors:
; ;
Publication Date:
Research Org.:
Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source
Sponsoring Org.:
Doe - Office Of Science
OSTI Identifier:
913907
Report Number(s):
BNL-78475-2007-JA
Journal ID: ISSN 0027-8424; PNASA6; TRN: US0801400
DOE Contract Number:  
DE-AC02-98CH10886
Resource Type:
Journal Article
Journal Name:
Proc Natl Acad Sci USA
Additional Journal Information:
Journal Volume: 102; Journal ID: ISSN 0027-8424
Country of Publication:
United States
Language:
English
Subject:
43 PARTICLE ACCELERATORS; AMINO ACIDS; DNA; GENES; PROMOTERS; PROTEINS; RESIDUES; RNA POLYMERASES; SYNCHROTRONS; TRANSCRIPTION; national synchrotron light source

Citation Formats

Brenowitz, M, Erie, D, and Chance, M. Catching RNA Polymerase in the act of Binding: Intermediates in Transcription Illuminated by Synchrotron Footprinting. United States: N. p., 2005. Web. doi:10.1073/pnas.0501152102.
Brenowitz, M, Erie, D, & Chance, M. Catching RNA Polymerase in the act of Binding: Intermediates in Transcription Illuminated by Synchrotron Footprinting. United States. https://doi.org/10.1073/pnas.0501152102
Brenowitz, M, Erie, D, and Chance, M. 2005. "Catching RNA Polymerase in the act of Binding: Intermediates in Transcription Illuminated by Synchrotron Footprinting". United States. https://doi.org/10.1073/pnas.0501152102.
@article{osti_913907,
title = {Catching RNA Polymerase in the act of Binding: Intermediates in Transcription Illuminated by Synchrotron Footprinting},
author = {Brenowitz, M and Erie, D and Chance, M},
abstractNote = {The article by Sclavi et al. in this issue of PNAS addresses 'initiation, ' the first step in transcription. Gene transcription is catalyzed in cells by large multisubunit proteins called RNA polymerases (RNAP). The eubacteria holoenzyme of RNAP is composed of five core subunits ({alpha}, {alpha}2, {beta}, {beta}', and {omega}) that contain the amino acid residues required for the enzyme's catalytic activity. A sixth subunit ({sigma}) guides RNAP to specific sequences on the genomic DNA (promoters) that mark the beginning of a gene or group of genes.},
doi = {10.1073/pnas.0501152102},
url = {https://www.osti.gov/biblio/913907}, journal = {Proc Natl Acad Sci USA},
issn = {0027-8424},
number = ,
volume = 102,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 2005},
month = {Sat Jan 01 00:00:00 EST 2005}
}