Activity-dependent Fluorescent Labeling of Bacteria that Degrade Toluene via 3-methylcatechol
Journal Article
·
· Journal of Microbiological Methods
3-Hydroxyphenylacetylene (3-HPA) served as a novel, activity-dependent, fluorogenic and chromogenic probe for bacterial enzymes known to degrade toluene via meta ring fission of the intermediate, 3-methylcatechol. By this direct physiological analysis, cells grown with an aromatic substrate to induce the synthesis of toluene-degrading enzymes were fluorescently labeled.
- Research Organization:
- Idaho National Lab. (INL), Idaho Falls, ID (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- DE-AC07-99ID-13727
- OSTI ID:
- 911977
- Report Number(s):
- INEEL/JOU-02-01460; JMIMDQ; TRN: US200801%%422
- Journal Information:
- Journal of Microbiological Methods, Journal Name: Journal of Microbiological Methods; ISSN 0167-7012
- Country of Publication:
- United States
- Language:
- English
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