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Title: A New DNA Binding Protein Highly Conserved in Diverse Crenarchaeal Viruses

Abstract

Sulfolobus turreted icosahedral virus (STIV) infects Sulfolobus species found in the hot springs of Yellowstone National Park. Its 37 open reading frames (ORFs) generally lack sequence similarity to other genes. One exception, however, is ORF B116. While its function is unknown, orthologs are found in three additional crenarchaeal viral families. Due to the central importance of this protein family to crenarchaeal viruses, we have undertaken structural and biochemical studies of B116. The structure reveals a previously unobserved fold consisting of a five-stranded beta-sheet flanked on one side by three alpha helices. Two subunits come together to form a homodimer with a 10-stranded mixed beta-sheet, where the topology of the central strands resembles an unclosed beta-barrel. Highly conserved loops rise above the surface of the saddle-shaped protein and suggest an interaction with the major groove of DNA. The predicted B116-DNA interaction is confirmed by electrophoretic mobility shift assays.

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
909818
Report Number(s):
SLAC-REPRINT-2007-076
Journal ID: ISSN 0042-6822; VIRLAX; TRN: US200723%%208
DOE Contract Number:  
AC02-76SF00515
Resource Type:
Journal Article
Journal Name:
Virology 363:387-396,2007
Additional Journal Information:
Journal Volume: 363; Journal ID: ISSN 0042-6822
Country of Publication:
United States
Language:
English
Subject:
15 GEOTHERMAL ENERGY; DNA; GENES; HOT SPRINGS; PROTEINS; VIRUSES; DIMERS; BIOCHEMISTRY; Other,OTHER

Citation Formats

Larson, E T, Eilers, B J, Reiter, D, Ortmann, A C, Young, M J, Lawrence, C M, and /Montana State U. /Tubingen U. A New DNA Binding Protein Highly Conserved in Diverse Crenarchaeal Viruses. United States: N. p., 2007. Web. doi:10.1016/j.virol.2007.01.027.
Larson, E T, Eilers, B J, Reiter, D, Ortmann, A C, Young, M J, Lawrence, C M, & /Montana State U. /Tubingen U. A New DNA Binding Protein Highly Conserved in Diverse Crenarchaeal Viruses. United States. https://doi.org/10.1016/j.virol.2007.01.027
Larson, E T, Eilers, B J, Reiter, D, Ortmann, A C, Young, M J, Lawrence, C M, and /Montana State U. /Tubingen U. 2007. "A New DNA Binding Protein Highly Conserved in Diverse Crenarchaeal Viruses". United States. https://doi.org/10.1016/j.virol.2007.01.027.
@article{osti_909818,
title = {A New DNA Binding Protein Highly Conserved in Diverse Crenarchaeal Viruses},
author = {Larson, E T and Eilers, B J and Reiter, D and Ortmann, A C and Young, M J and Lawrence, C M and /Montana State U. /Tubingen U.},
abstractNote = {Sulfolobus turreted icosahedral virus (STIV) infects Sulfolobus species found in the hot springs of Yellowstone National Park. Its 37 open reading frames (ORFs) generally lack sequence similarity to other genes. One exception, however, is ORF B116. While its function is unknown, orthologs are found in three additional crenarchaeal viral families. Due to the central importance of this protein family to crenarchaeal viruses, we have undertaken structural and biochemical studies of B116. The structure reveals a previously unobserved fold consisting of a five-stranded beta-sheet flanked on one side by three alpha helices. Two subunits come together to form a homodimer with a 10-stranded mixed beta-sheet, where the topology of the central strands resembles an unclosed beta-barrel. Highly conserved loops rise above the surface of the saddle-shaped protein and suggest an interaction with the major groove of DNA. The predicted B116-DNA interaction is confirmed by electrophoretic mobility shift assays.},
doi = {10.1016/j.virol.2007.01.027},
url = {https://www.osti.gov/biblio/909818}, journal = {Virology 363:387-396,2007},
issn = {0042-6822},
number = ,
volume = 363,
place = {United States},
year = {Mon Jul 09 00:00:00 EDT 2007},
month = {Mon Jul 09 00:00:00 EDT 2007}
}