Nano-Biotechnology in Using Enzymes for Environmental Remediation: Single-Enzyme Nanoparticles
Book
·
OSTI ID:909688
We have developed armored single-enzyme nanoparticles (SENs), which dramatically stabilize a protease (a-chymotrypsin, CT) by surrounding each enzyme molecule with a porous composite organic/inorganic shell of less than a few nanometers thick. The armored enzymes show no decrease in CT activity at 30°C for a day while free CT activity is rapidly reduced by orders of magnitude. The armored shell around CT is sufficiently thin and porous that it does not place any serious mass-transfer limitation of substrate. This unique approach will have a great impact in using enzymes in various fields, including environmental remediation.
- Research Organization:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 909688
- Report Number(s):
- PNNL-SA-39368; 3241; KP1301020; TRN: US200723%%10
- Resource Relation:
- Related Information: ACS Symposium Series, Nanotechnology and the Environment: Applications and Implications, 890:220-225
- Country of Publication:
- United States
- Language:
- English
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