Bac clones generated from sheared dna
BAC libraries generated from restriction-digested genomic DNA display representational bias and lack some sequences. To facilitate completion of genome projects, procedures have been developed to create BACs from DNA physically sheared to create fragments extending up to 200kb. The DNA fragments were repaired to create blunt ends and ligated to a new BAC vector. This approach has been tested by generating BAC libraries from Drosophila DNA, with insert lengths of 50 kb to 150 kb. The libraries lack chimeric clone problems as determined by mapping paired BAC-end sequences of one library to the D. melanogaster genome sequence. The utility of ''sheared'' libraries was demonstrated by closure of a previous clone gap and by isolation of clones from telomeric regions, which were notably absent from previous Drosophila BAC libraries.
- Research Organization:
- COLLABORATION - Children s Hospital andResearch Center at Oakland, Oakland, CA
- DOE Contract Number:
- DE-AC02-05CH11231
- OSTI ID:
- 899797
- Report Number(s):
- LBNL-61520; GNMCEP; R&D Project: L0001 (EXPIRED); TRN: US200708%%537
- Journal Information:
- Genomics, Vol. 89, Issue 2; Related Information: Journal Publication Date: 2007; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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