Fluorescence in situ hybridization and spectral imaging analysisof human oocytes and first polar bodies

Journal Article · Wed Oct 06 00:00:00 EDT 2004 · Journal of Histochemistry and Cytochemistry

OSTI ID:878309

Weier, Heinz-Ulli G; Weier, Jingly F; Oter Renom, Maria; Zheng, Xuezhong; Colls, Pere; Nureddin, Aida; Pham, Chau D; Chu, Lisa W; Racowsky, Catherine; Munne, Santiago

We investigated the frequencies of abnormalities involving either chromosome 1, 16, 18 or 21 in failed-fertilized human oocytes.While abnormalities involving chromosome 16 showed an age-dependant increase, results for the other chromosomes did not show statistically significant differences between the three age groups <35 yrs, 35-39 yrs, and >39 yrs. The scoring of four chromosomes is likely to underestimate the true rate of aneuploid cells. Thus, for a pilot study investigating a more comprehensive analysis of oocytes and their corresponding first polar bodies (1PBs), we developed a novel 8-probe chromosome enumeration scheme using FISH and SIm.

• Research Organization: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
• Sponsoring Organization: USDOE; US National Institutes of Health Grant1-R44-HD-44313; Brigham and Women's Hospital Ob/Gyn IVF DiscretionaryFunds,Industry Grant 0083
• DOE Contract Number: DE-AC02-05CH11231
• OSTI ID: 878309
• Report Number(s): LBNL-55393; JHCYAS; R&D Project: L0083; BnR: 600301010; TRN: US200611%%120
• Journal Information: Journal of Histochemistry and Cytochemistry, Vol. 53, Issue 3; Related Information: Journal Publication Date: 01/03/2005; ISSN 0022-1554
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AGE GROUPS
CHROMOSOMES
FLUORESCENCE
IN-SITU HYBRIDIZATION
OOCYTES
Aneuploidy oocytes polar bodies fertilization cytogeneticsfluorescence in situ hybridization