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Title: Automated sample mounting and technical advance alignment system for biological crystallography at a synchrotron source

Abstract

High-throughput data collection for macromolecular crystallography requires an automated sample mounting system for cryo-protected crystals that functions reliably when integrated into protein-crystallography beamlines at synchrotrons. Rapid mounting and dismounting of the samples increases the efficiency of the crystal screening and data collection processes, where many crystals can be tested for the quality of diffraction. The sample-mounting subsystem has random access to 112 samples, stored under liquid nitrogen. Results of extensive tests regarding the performance and reliability of the system are presented. To further increase throughput, we have also developed a sample transport/storage system based on ''puck-shaped'' cassettes, which can hold sixteen samples each. Seven cassettes fit into a standard dry shipping Dewar. The capabilities of a robotic crystal mounting and alignment system with instrumentation control software and a relational database allows for automated screening and data collection to be developed.

Authors:
; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE; National Institutes of Health. General Medical Sciences. Protein Structure Initiative Grants GM62648-03 and GM62412-04 and GM064655-01 (US)
OSTI Identifier:
837930
Report Number(s):
LBNL-54790
R&D Project: 862S1B; TRN: US0501166
DOE Contract Number:  
AC03-76SF00098
Resource Type:
Journal Article
Journal Name:
Structure
Additional Journal Information:
Journal Volume: 12; Journal Issue: 4; Other Information: Journal Publication Date: 04/2004; PBD: 7 Jan 2004
Country of Publication:
United States
Language:
English
Subject:
43 PARTICLE ACCELERATORS; 47 OTHER INSTRUMENTATION; 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; ALIGNMENT; CRYSTALLOGRAPHY; DIFFRACTION; EFFICIENCY; NITROGEN; PERFORMANCE; RELIABILITY; SYNCHROTRONS; ROBOTICS BIOLOGICAL CRYSTALLOGRAPHY SYNCHROTRON

Citation Formats

Snell, Gyorgy, Cork, Carl, Nordmeyer, Robert, Cornell, Earl, Meigs, George, Yegian, Derek, Jaklevic, Joseph, Jin, Jian, Stevens, Raymond C, and Earnest, Thomas. Automated sample mounting and technical advance alignment system for biological crystallography at a synchrotron source. United States: N. p., 2004. Web. doi:10.1016/j.str.2004.03.011.
Snell, Gyorgy, Cork, Carl, Nordmeyer, Robert, Cornell, Earl, Meigs, George, Yegian, Derek, Jaklevic, Joseph, Jin, Jian, Stevens, Raymond C, & Earnest, Thomas. Automated sample mounting and technical advance alignment system for biological crystallography at a synchrotron source. United States. https://doi.org/10.1016/j.str.2004.03.011
Snell, Gyorgy, Cork, Carl, Nordmeyer, Robert, Cornell, Earl, Meigs, George, Yegian, Derek, Jaklevic, Joseph, Jin, Jian, Stevens, Raymond C, and Earnest, Thomas. 2004. "Automated sample mounting and technical advance alignment system for biological crystallography at a synchrotron source". United States. https://doi.org/10.1016/j.str.2004.03.011.
@article{osti_837930,
title = {Automated sample mounting and technical advance alignment system for biological crystallography at a synchrotron source},
author = {Snell, Gyorgy and Cork, Carl and Nordmeyer, Robert and Cornell, Earl and Meigs, George and Yegian, Derek and Jaklevic, Joseph and Jin, Jian and Stevens, Raymond C and Earnest, Thomas},
abstractNote = {High-throughput data collection for macromolecular crystallography requires an automated sample mounting system for cryo-protected crystals that functions reliably when integrated into protein-crystallography beamlines at synchrotrons. Rapid mounting and dismounting of the samples increases the efficiency of the crystal screening and data collection processes, where many crystals can be tested for the quality of diffraction. The sample-mounting subsystem has random access to 112 samples, stored under liquid nitrogen. Results of extensive tests regarding the performance and reliability of the system are presented. To further increase throughput, we have also developed a sample transport/storage system based on ''puck-shaped'' cassettes, which can hold sixteen samples each. Seven cassettes fit into a standard dry shipping Dewar. The capabilities of a robotic crystal mounting and alignment system with instrumentation control software and a relational database allows for automated screening and data collection to be developed.},
doi = {10.1016/j.str.2004.03.011},
url = {https://www.osti.gov/biblio/837930}, journal = {Structure},
number = 4,
volume = 12,
place = {United States},
year = {Wed Jan 07 00:00:00 EST 2004},
month = {Wed Jan 07 00:00:00 EST 2004}
}