Induction of transpositions of MGE Dm412 by {gamma}-radiation in an isogenic line of Drosophila melanogaster

Zabanov, S A; Vasil`eva, L A; Ratner, V A

Title: Induction of transpositions of MGE Dm412 by {gamma}-radiation in an isogenic line of Drosophila melanogaster

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Abstract

In an isogenic line of Drosophila, transpositions of mobile genetic elements (MGE) Dm412 were induced by {gamma}-radiation at doses of 300, 800, and 1300 R. The rates of induced transpositions were (for each dose, respectively) 3.9 x 10{sup {minus}3}, 1.0 x 10{sup {minus}2}, and 1.87 x 10{sup {minus}2} events per occupied site per haploid genome of the isogenic line per generation. Thus, the transposition rate increased linearly with the radiation dose. The specific rate of {gamma}-radiation-induced transpositions was (1.3 {+-} 0.6) x 10{sup {minus}5} per occupied site per haploid genome of the isogenic line per Roentgen per generation. {gamma}-Radiation-induced hot transposition sites and haplotypes, very similar to those induced by heat shock, were found. It was suggested that the mechanism of induction by {gamma}-radiation involves the heat shock system. Thus, it is more similar to the mechanism of temperature induction than to the direct mutational effect of {gamma}-radiation. Estimates of induced transposition rates per genome for each dose were calculated as 1.1, 3.0, and 5.6 events, respectively, per genome per generation. This level probably corresponds to the subthreshold level of genomes near the {open_quotes}catastrophic border of transpositional losses.{close_quotes} 21 refs., 1 fig., 4 tabs.

Authors:

Zabanov, S A ^[1]; Vasil`eva, L A; Ratner, V A ^[1]

Institute of Cytology and Genetics, Novosibirsk (Russian Federation)

Publication Date:: Thu Jun 01 00:00:00 EDT 1995

OSTI Identifier:: 83447

Resource Type:: Journal Article

Journal Name:: Russian Journal of Genetics

Additional Journal Information:: Journal Volume: 31; Journal Issue: 6; Other Information: PBD: Jun 1995; TN: Translated from Genetika; 31: No. 6, 798-803(1995)

Country of Publication:: United States

Language:: English

Subject:: 56 BIOLOGY AND MEDICINE, APPLIED STUDIES; 55 BIOLOGY AND MEDICINE, BASIC STUDIES; DROSOPHILA; BIOLOGICAL STRESS; GENE MUTATIONS; CHROMOSOMES; GENETIC RADIATION EFFECTS; GENES; GAMMA RADIATION; DNA HYBRIDIZATION

Citation Formats


                    Zabanov, S A, Vasil`eva, L A, Ratner, V A, and Novosibirsk State Univ. Induction of transpositions of MGE Dm412 by {gamma}-radiation in an isogenic line of Drosophila melanogaster.  United States: N. p., 1995. 
        Web.

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                    Zabanov, S A, Vasil`eva, L A, Ratner, V A, & Novosibirsk State Univ. Induction of transpositions of MGE Dm412 by {gamma}-radiation in an isogenic line of Drosophila melanogaster.  United States.

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                    Zabanov, S A, Vasil`eva, L A, Ratner, V A, and Novosibirsk State Univ. 1995.  
        "Induction of transpositions of MGE Dm412 by {gamma}-radiation in an isogenic line of Drosophila melanogaster".  United States.

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@article{osti_83447,

  title        = {Induction of transpositions of MGE Dm412 by {gamma}-radiation in an isogenic line of Drosophila melanogaster},

  author       = {Zabanov, S A and Vasil`eva, L A and Ratner, V A and Novosibirsk State Univ.},

  abstractNote = {In an isogenic line of Drosophila, transpositions of mobile genetic elements (MGE) Dm412 were induced by {gamma}-radiation at doses of 300, 800, and 1300 R. The rates of induced transpositions were (for each dose, respectively) 3.9 x 10{sup {minus}3}, 1.0 x 10{sup {minus}2}, and 1.87 x 10{sup {minus}2} events per occupied site per haploid genome of the isogenic line per generation. Thus, the transposition rate increased linearly with the radiation dose. The specific rate of {gamma}-radiation-induced transpositions was (1.3 {+-} 0.6) x 10{sup {minus}5} per occupied site per haploid genome of the isogenic line per Roentgen per generation. {gamma}-Radiation-induced hot transposition sites and haplotypes, very similar to those induced by heat shock, were found. It was suggested that the mechanism of induction by {gamma}-radiation involves the heat shock system. Thus, it is more similar to the mechanism of temperature induction than to the direct mutational effect of {gamma}-radiation. Estimates of induced transposition rates per genome for each dose were calculated as 1.1, 3.0, and 5.6 events, respectively, per genome per generation. This level probably corresponds to the subthreshold level of genomes near the {open_quotes}catastrophic border of transpositional losses.{close_quotes} 21 refs., 1 fig., 4 tabs.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/83447},
  journal      = {Russian Journal of Genetics},
number       = 6,

  volume       = 31,

  place        = {United States},

  year         = {Thu Jun 01 00:00:00 EDT 1995},

  month        = {Thu Jun 01 00:00:00 EDT 1995}

}

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