Single nucleotide polymorphism markers for genetic mapping in Drosophila melanogaster
Abstract
For nearly a century, genetic analysis in Drosophila melanogaster has been a powerful tool for analyzing gene function, yet Drosophila lacks the molecular genetic mapping tools that have recently revolutionized human, mouse and plant genetics. Here, we describe the systematic characterization of a dense set of molecular markers in Drosophila using an STS-based physical map of the genome. We identify 474 biallelic markers in standard laboratory strains of Drosophila that the genome. The majority of these markers are single nucleotide polymorphisms (SNPs) and sequences for these variants are provided in an accessible format. The average density of the new markers is 1 marker per 225 kb on the autosomes and 1 marker per 1 Mb on the X chromosome. We include in this survey a set of P-element strains that provide additional utility for high-resolution mapping. We demonstrate one application of the new markers in a simple set of crosses to map a mutation in the hedgehog gene to an interval of <1 Mb. This new map resource significantly increases the efficiency and resolution of recombination mapping and will be of immediate value to the Drosophila research community.
- Authors:
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- National Institutes of Health (US)
- OSTI Identifier:
- 785285
- Report Number(s):
- LBNL-47759
R&D Project: 803NBE; TRN: AH200131%%160
- DOE Contract Number:
- AC03-76SF00098
- Resource Type:
- Journal Article
- Journal Name:
- Genome Research
- Additional Journal Information:
- Journal Volume: 11; Journal Issue: 6; Other Information: Journal Publication Date: June 2001; PBD: 16 Apr 2001
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; DROSOPHILA; EFFICIENCY; GENES; GENETIC MAPPING; GENETICS; MUTATIONS; NUCLEOTIDES; RECOMBINATION; RESOLUTION; STRAINS; X CHROMOSOME; DROSOPHILA SNP GENETIC MAPPING
Citation Formats
Hoskins, Roger A, Phan, Alexander C, Naeemuddin, Mohammed, Mapa, Felipa A, Ruddy, David A, Ryan, Jessica J, Young, Lynn M, Wells, Trent, Kopczynski, Casey, and Ellis, Michael C. Single nucleotide polymorphism markers for genetic mapping in Drosophila melanogaster. United States: N. p., 2001.
Web. doi:10.1101/gr.GR-1780R.
Hoskins, Roger A, Phan, Alexander C, Naeemuddin, Mohammed, Mapa, Felipa A, Ruddy, David A, Ryan, Jessica J, Young, Lynn M, Wells, Trent, Kopczynski, Casey, & Ellis, Michael C. Single nucleotide polymorphism markers for genetic mapping in Drosophila melanogaster. United States. https://doi.org/10.1101/gr.GR-1780R
Hoskins, Roger A, Phan, Alexander C, Naeemuddin, Mohammed, Mapa, Felipa A, Ruddy, David A, Ryan, Jessica J, Young, Lynn M, Wells, Trent, Kopczynski, Casey, and Ellis, Michael C. 2001.
"Single nucleotide polymorphism markers for genetic mapping in Drosophila melanogaster". United States. https://doi.org/10.1101/gr.GR-1780R.
@article{osti_785285,
title = {Single nucleotide polymorphism markers for genetic mapping in Drosophila melanogaster},
author = {Hoskins, Roger A and Phan, Alexander C and Naeemuddin, Mohammed and Mapa, Felipa A and Ruddy, David A and Ryan, Jessica J and Young, Lynn M and Wells, Trent and Kopczynski, Casey and Ellis, Michael C},
abstractNote = {For nearly a century, genetic analysis in Drosophila melanogaster has been a powerful tool for analyzing gene function, yet Drosophila lacks the molecular genetic mapping tools that have recently revolutionized human, mouse and plant genetics. Here, we describe the systematic characterization of a dense set of molecular markers in Drosophila using an STS-based physical map of the genome. We identify 474 biallelic markers in standard laboratory strains of Drosophila that the genome. The majority of these markers are single nucleotide polymorphisms (SNPs) and sequences for these variants are provided in an accessible format. The average density of the new markers is 1 marker per 225 kb on the autosomes and 1 marker per 1 Mb on the X chromosome. We include in this survey a set of P-element strains that provide additional utility for high-resolution mapping. We demonstrate one application of the new markers in a simple set of crosses to map a mutation in the hedgehog gene to an interval of <1 Mb. This new map resource significantly increases the efficiency and resolution of recombination mapping and will be of immediate value to the Drosophila research community.},
doi = {10.1101/gr.GR-1780R},
url = {https://www.osti.gov/biblio/785285},
journal = {Genome Research},
number = 6,
volume = 11,
place = {United States},
year = {Mon Apr 16 00:00:00 EDT 2001},
month = {Mon Apr 16 00:00:00 EDT 2001}
}