Isolation of nine gene sequences induced by silica in murine macrophages

Segade, F; Claudio, E; Wrobel, K; Ramos, S; Lazo, P S

Title: Isolation of nine gene sequences induced by silica in murine macrophages

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Abstract

Macrophage activation by silica is the initial step in the development of silicosis. To identify genes that might be involved in silica-mediated activation, RAW 264.7 mouse macrophages were treated with silica for 48 h, and a subtracted cDNA library enriched for silica-induced genes (SIG) was constructed and differently screened. Nine cDNA clones (designated SIG-12, -14, -20, -41, -61, -81, -91, and -111) were partially sequenced and compared with sequences in GenBank/EMBL databases. SIG-12, -14, and -20 corresponded to the genes for ribosomal proteins L13A, L32, and L26, respectively. SIG-61 is the mouse homologue of p21 RhoC. SIG-91 is identical to the 67-kDa high-affinity laminin receptor. Four genes were not identified and are novel. All of the mRNAs corresponding to the nine cloned cDNAs were inducible by silica. Steady-state levels of mRNAs in RAW 264.7 cells treated with various macrophage activators and inducers of signal transduction pathways were determined. A complex pattern of induction and repression was found, indicating that upon phagocytosis of silica particles, many regulatory mechanisms of genes expression are simultaneously triggered. 55 refs., 4 figs., 1 tab.

Authors:

Segade, F; Claudio, E; Wrobel, K; Ramos, S; Lazo, P S ^[1]

Oviedo Univ., Oviedo (Spain)

Publication Date:: Wed Mar 01 00:00:00 EST 1995

OSTI Identifier:: 75585

Resource Type:: Journal Article

Journal Name:: Journal of Immunology

Additional Journal Information:: Journal Volume: 154; Journal Issue: 5; Other Information: PBD: 1 Mar 1995

Country of Publication:: United States

Language:: English

Subject:: 55 BIOLOGY AND MEDICINE, BASIC STUDIES; SILICA; INHALATION; MACROPHAGES; CHEMICAL ACTIVATION; ANIMAL CELLS; DNA SEQUENCING; DNA-CLONING; GENE REGULATION; PNEUMOCONIOSES; MICE; MESSENGER-RNA; PHAGOCYTOSIS; BANDING TECHNIQUES

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                    Segade, F, Claudio, E, Wrobel, K, Ramos, S, and Lazo, P S. Isolation of nine gene sequences induced by silica in murine macrophages.  United States: N. p., 1995. 
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                    Segade, F, Claudio, E, Wrobel, K, Ramos, S, & Lazo, P S. Isolation of nine gene sequences induced by silica in murine macrophages.  United States.

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                    Segade, F, Claudio, E, Wrobel, K, Ramos, S, and Lazo, P S. 1995.  
        "Isolation of nine gene sequences induced by silica in murine macrophages".  United States.

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@article{osti_75585,

  title        = {Isolation of nine gene sequences induced by silica in murine macrophages},

  author       = {Segade, F and Claudio, E and Wrobel, K and Ramos, S and Lazo, P S},

  abstractNote = {Macrophage activation by silica is the initial step in the development of silicosis. To identify genes that might be involved in silica-mediated activation, RAW 264.7 mouse macrophages were treated with silica for 48 h, and a subtracted cDNA library enriched for silica-induced genes (SIG) was constructed and differently screened. Nine cDNA clones (designated SIG-12, -14, -20, -41, -61, -81, -91, and -111) were partially sequenced and compared with sequences in GenBank/EMBL databases. SIG-12, -14, and -20 corresponded to the genes for ribosomal proteins L13A, L32, and L26, respectively. SIG-61 is the mouse homologue of p21 RhoC. SIG-91 is identical to the 67-kDa high-affinity laminin receptor. Four genes were not identified and are novel. All of the mRNAs corresponding to the nine cloned cDNAs were inducible by silica. Steady-state levels of mRNAs in RAW 264.7 cells treated with various macrophage activators and inducers of signal transduction pathways were determined. A complex pattern of induction and repression was found, indicating that upon phagocytosis of silica particles, many regulatory mechanisms of genes expression are simultaneously triggered. 55 refs., 4 figs., 1 tab.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/75585},
  journal      = {Journal of Immunology},
number       = 5,

  volume       = 154,

  place        = {United States},

  year         = {Wed Mar 01 00:00:00 EST 1995},

  month        = {Wed Mar 01 00:00:00 EST 1995}

}

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