Characterization of a novel HLA-A2 variant, A*0214, by ARMS-PCR and DNA sequencing
- Institute of Molecular Medicine, Headington, Oxford (United Kingdom)
With recent advances in DNA-based methods for typing HLA class I alleles, the discrimination of new variants not readily detectable by serological or biochemical means has become possible. We used an ARMS polymerase chain reaction (PCR)-based system that identifies HLA-A locus alleles to high resolution to type a Kenyan Black African individual. The unexpected result was indicative of a novel HLA-A*02 allele. Genomic DNA from this individual was analyzed by ARMS-PCR low-resolution typing, high resolution subtyping, and PCR gene mapping. Low-resolution typing identified the sample as HLA-A*02, A*33. High-resolution HLA-A*02 subtyping (data not published), however, suggested the sample was A*02 heterozygous (A*0205, A*0206). In view of the low-resolution HLA-A locus typing, this raised the possibility that the A*02 gene was a new HLA-A*02 variant that contained sequence motifs characteristic of both the A*0205 and A*0206 alleles. 6 refs., 1 fig., 2 tabs.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 75575
- Journal Information:
- Immunogenetics, Vol. 41, Issue 1; Other Information: PBD: Jan 1995
- Country of Publication:
- United States
- Language:
- English
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