Osmotic pressures and second virial coefficients for aqueous saline solutions of lysozyme

Journal Article · Mon Mar 27 00:00:00 EST 2000 · Fluid Phase Equilibria

DOI:https://doi.org/10.1016/S0378-3812(99)00337-4· OSTI ID:751781

Moon, Y. U. ^[1]; Anderson, C. O. ^[1]; Blanch, H. W. ^[1]; Prausnitz, J. M. ^[2]

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1. Univ. of California, Berkeley, CA (United States)
2. Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Experimental data at 25 °C are reported for osmotic pressures of aqueous solutions containing lysozyme and any one of the following salts: ammonium sulfate, ammonium oxalate and ammonium phosphate at ionic strength 1 or 3M. Data were obtained using a Wescor Colloid Membrane Osmometer at lysozyme concentrations from about 4 to 20 grams per liter at pH 4, 7 or 8. Osmotic second virial coefficients for lysozyme were calculated from the osmotic-pressure data. All coefficients were negative, increasing in magnitude with ionic strength. Furthermore, tesults are insensitive to the nature of the anion, but rise slightly in magnitude as the size of the anion increases.

• Research Organization: Lawrence Berkeley National Laboratory, Berkeley, CA (United States)
• Sponsoring Organization: USDOE Office of Science (SC), Basic Energy Sciences (BES)
• Grant/Contract Number: AC03-76SF00098
• OSTI ID: 751781
• Report Number(s): LBNL-44257; R&D Project: 402201
• Journal Information: Fluid Phase Equilibria, Vol. 168, Issue 2; ISSN 0378-3812
• Publisher: Elsevier
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
proteins
salts
intermolecular interactions
potentials of mean force
osmotic-pressure data
experimental method