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Title: Molecular ecology of bacterial populations in environmental hazardous chemical control. Annual report, 15 January 1993-14 January 1994

Technical Report ·
OSTI ID:7286288

The major outcomes of the current work are: (1) Development of a new molecular strategy, mRNA extraction from soil, assesses the catabolic activity of soil bacteria in situ. (2) Quantitative the association between the biosensor bioluminescence response and the PAHs bioavailability present in the waste environment. (3) Demonstration the ability of NAH plasmid to mediate the initial biodegradation reactions in the catabolic pathway of fluorence. The current research work is focuses on developing new molecular diagnostics' method for measuring in situ PAH biodegradation activity and co-related the bioluminescence response, that produced by a naphthalene-lux reporter strain, to the bioavailability of different pollutants in the real environment. In addition, catabolism of a tricyclic aromatic hydrocarbon, fluorene, mediates by a NAH plasmid is also investigated.

Research Organization:
Tennessee Univ., Knoxville, TN (United States). Center for Environmental Biotechnology
OSTI ID:
7286288
Report Number(s):
AD-A-278340/5/XAB; CNN: F49620-92-J-0147
Country of Publication:
United States
Language:
English