Gel-based Indo-1 probe for monitoring calcium(II) ions

Journal Article · Tue Mar 15 00:00:00 EST 1994 · Analytical Chemistry (Washington); (United States)

DOI:https://doi.org/10.1021/ac00078a009· OSTI ID:7267333

Vo-Dinh, T; Ramirez, L; Pal, A ^[1]; Viallet, P ^[2]

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1. Oak Ridge National Lab., TN (United States)
2. Univ. of Perpignan (France)

This work describes the development of a new gel-based Indo-1 (GBI) fluorescent probe for use in detection of Ca[sup 2+]. The carbodiimide-binding procedure, which allows the formation of an amide bond between the aromatic carboxylic group of Indo-1 and an amino group of the gel has been selected for this study. In this work, agarose gel, which has an amino terminal group with a six-atom hydrophilic spacer arm, has been chosen for hosting Indo-1. A phase-transfer catalyst was used to allow efficient diffusion of metal ions into the GBI probe. The results indicate that the GBI probe is an efficient and practical system that has the required sensitivity to detect Ca[sup 2+] ions in biological fluids for routine monitoring. 23 refs., 7 figs.

• DOE Contract Number: AC05-84OR21400
• OSTI ID: 7267333
• Journal Information: Analytical Chemistry (Washington); (United States), Vol. 66:6; ISSN 0003-2700
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
CALCIUM IONS
DIFFUSION
MONITORING
AROMATICS
BODY FLUIDS
CATALYSTS
CHEMICAL BONDS
FLUORESCENCE
GELS
PH VALUE
PROBES
SACCHARIDES
BIOLOGICAL MATERIALS
CARBOHYDRATES
CHARGED PARTICLES
COLLOIDS
DISPERSIONS
IONS
LUMINESCENCE
MATERIALS
ORGANIC COMPOUNDS
400201* - Chemical & Physicochemical Properties
400500 - Photochemistry
550200 - Biochemistry