Studies on the relationship between epidermal cell turnover kinetics and permeability of hairless mouse skin

Thesis/Dissertation · Fri Jan 01 00:00:00 EST 1988

OSTI ID:7266631

Han, S R

The primary aim of this study was to develop non-invasive, physical means to quantitatively assess the epidermal turnover kinetics and barrier properties of the skin and relate these to the cutaneous irritation which results from ultraviolet light irradiation and mold thermal burns. After systematically injecting radiolabeled glycine, the appearance of radioactivity at the skin's surface indicated the transit time of radiolabeled cells through the skin. By plotting the data as the cumulative specific activity against time and then fitting them with a third order polynomial equation, it is possible to estimate the turnover time of the stratum corneum. The skin turnover was coordinated with non-invasive transepidermal water loss (TEWL) studies determined with an evaporimeter. In vitro diffusion studies of the permeability of hydrocortisone through UVB irradiated and thermally burned skin were also performed. The studies indicated that irritated skin offers a relatively low diffusional resistance to hydrocortisone. Depending on the severity of the trauma, the increases in hydrocortisone's permeability coefficient through irritated skin ranged from a low of about 2 times normal to a high of about 210 times normal. Trauma-induced changes in hydrocortisone permeability parallel changes in TEWL, proving that the barrier deficient state resulting from rapid epidermal turnover is a general phenomenon.

• Research Organization: Michigan Univ., Ann Arbor, MI (USA)
• OSTI ID: 7266631
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
EPIDERMIS
BIOLOGICAL RADIATION EFFECTS
WOUNDS
HEALING
BURNS
HYDROCORTISONE
MICE
PERMEABILITY
RADIOISOTOPES
TRACER TECHNIQUES
ULTRAVIOLET RADIATION
ADRENAL HORMONES
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BODY
CORTICOSTEROIDS
ELECTROMAGNETIC RADIATION
EPITHELIUM
GLUCOCORTICOIDS
HYDROXY COMPOUNDS
INJURIES
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
MAMMALS
ORGANIC COMPOUNDS
ORGANS
PREGNANES
RADIATION EFFECTS
RADIATIONS
RECOVERY
RODENTS
SKIN
STEROIDS
TISSUES
VERTEBRATES
550901* - Pathology- Tracer Techniques