Genetics of bacteria that utilize one-carbon compounds
Methylotrophic bacteria that grow on the one-carbon compounds; methane, methanol, methylamines and dichloromethane are a morphologically and physiologically diverse group of eubacteria. The 16S rRNA molecules of several gram-negative methylotrophs have been sequenced. Two phylogenetically related groups containing type I and type II methylotrophs have been identified. Each group contains two subgroups of bacteria. DNA probes homologous to 16S rRNA's of each group of methanotrophs have been synthesized and have been shown to hybridize only to the 16S rRNA's from target bacteria. We have mapped the positions of 15 genes controlling the synthesis of methanol dehydrogenase, cytochrome C{sub L} and other functions required for the oxidation of methanol to formaldehyde in three species of type II methanotrophs. We have isolated a DNA-binding protein that binds to a cloned 172 bp sequence that is located upstream from the MDH structural gene. The function of this protein in the regulation of MDH synthesis will be investigated. The gene encoding the methane monooxygenase B component of {ital Methylosinus trichosporium} OB3b has been cloned and expressed in {ital Escherichia coli}. We intend to clone and map all five genes required for the expression of soluble MMO activity in {ital M. trichosporium} OB3b and to study the regulation of their synthesis. 5 refs.
- Research Organization:
- Minnesota Univ., Navarre, MN (USA). Gray Freshwater Biological Inst.
- Sponsoring Organization:
- DOE/ER
- DOE Contract Number:
- FG02-88ER13862
- OSTI ID:
- 7259842
- Report Number(s):
- DOE/ER/13862-T1; ON: DE90008485
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
METHANE
CATABOLISM
METHANOTROPHIC BACTERIA
GENETIC VARIABILITY
OXIDOREDUCTASES
DNA-CLONING
BIOLOGICAL PATHWAYS
COSMIDS
DNA SEQUENCING
GENETIC MAPPING
KREBS CYCLE
METHANOL
MOLECULAR BIOLOGY
PLASMIDS
PROGRESS REPORT
ALCOHOLS
ALKANES
BACTERIA
BIOLOGICAL VARIABILITY
CELL CONSTITUENTS
CLONING
DNA HYBRIDIZATION
DOCUMENT TYPES
ENZYMES
HYBRIDIZATION
HYDROCARBONS
HYDROXY COMPOUNDS
MAPPING
METABOLISM
MICROORGANISMS
ORGANIC COMPOUNDS
STRUCTURAL CHEMICAL ANALYSIS
090900* - Biomass Fuels- Processing- (1990-)
550500 - Metabolism